The study investigates the ability of methanolic extract of Andrographis paniculata (MAP) to supplement polyvalent anti-snake venom (ASV) in inhibiting neurotoxic enzyme acetylcholinesterase (AChE) and ‘spreading factor’ hyaluronidase from Naja naja (N.N) venom. AChE and hyaluronidase activity were measured in 100 or 200 µg of crude venom, respectively, and designated as ‘control’. In Test Group I, enzyme assays were performed immediately after the addition of ASV/MAP/ASV + MAP to the venom. Inhibition of AChE by ASV (100–367 µg) was 12–17%, and of hyaluronidase (22–660 µg) was 33–41%. Under the same conditions, MAP (100–400 µg) inhibited AChE and hyaluronidase to the extent of 17–33% and 17–52%, respectively. When ASV (220 µg) and MAP (100–200 µg) were added together, AChE and hyaluronidase were inhibited to a greater extent from 39–63 to 36–44%, than when either of them was used alone. In Test Group 2, the venom was incubated with ASV/MAP/ASV + MAP for 10–30 min at 37 °C prior to the assay which enhanced AChE inhibition by 6%, 82% and 18% respectively, when compared to Test Group I. Though there was no change in inhibition of hyaluronidase in the presence of ASV, MAP could further increase the extent of inhibition by 27% and ASV + MAP upto 4%. In Test Group III, venom and substrate were incubated for 90 min and hyaluronidase activity was measured after the addition of inhibitors. Here, ASV + MAP caused increased inhibition by 69% compared to ASV alone. The study confirms the ability of phytochemicals in MAP to contribute to a multipronged strategy by supplementing, thereby augmenting the efficacy of ASV.

这项研究调查穿心莲的甲醇提取物（MAP）补充多价抗蛇毒（ASV）抑制眼镜蛇眼镜蛇中神经毒性酶乙酰胆碱酯酶（AChE）和“扩散因子”透明质酸酶的能力。（NN）毒液。在100或200 µg的粗毒液中分别测定了AChE和透明质酸酶的活性，并称为“对照”。在测试组I中，在向毒液中添加ASV / MAP / ASV + MAP后立即进行酶测定。ASV（100–367 µg）对AChE的抑制率为12–17％，透明质酸酶（22–660 µg）的抑制率为33–41％。在相同条件下，MAP（100-400 µg）抑制AChE和透明质酸酶的程度分别为17-33％和17-52％。当分别加入ASV（220 µg）和MAP（100–200 µg）时，与单独使用时相比，AChE和透明质酸酶的抑制率在39-63％到36-44％之间更大。在测试组2中，将毒液与ASV / MAP / ASV + MAP在37°C下孵育10–30分钟，然后进行测定，与测试组相比，该毒液分别将AChE抑制作用提高6％，82％和18％。一世。尽管在存在ASV的情况下透明质酸酶的抑制作用没有变化，但MAP可以将抑制程度进一步提高27％，而ASV + MAP则可以提高至4％。在试验组III中，将毒液和底物温育90分钟，并在添加抑制剂后测量透明质酸酶活性。在这里，与单独使用ASV相比，ASV + MAP导致抑制增加了69％。该研究证实了MAP中的植物化学物质通过补充而有助于多管齐下的策略，从而增强了ASV的功效。与单独使用ASV相比，ASV + MAP引起的抑制作用增加了69％。该研究证实了MAP中的植物化学物质通过补充而有助于多管齐下的策略，从而增强了ASV的功效。与单独使用ASV相比，ASV + MAP引起的抑制作用增加了69％。该研究证实了MAP中的植物化学物质通过补充而有助于多管齐下的策略，从而增强了ASV的功效。