Genetic markers are widely used in fisheries management around the world. While the genetic structure and markers selected are usually based on samples from the wild, very few controlled experiments have been carried out to investigate possible differences in influence on traits between markers. Here we examine the bi-allelic gene pantophysin (Pan I), widely used in the management of Atlantic cod, in a series of in vitro crosses under a range of temperatures. It has been proposed that this gene, or another tightly linked gene, may be under strong divergent selection. Resolving this issue is essential in order to interpret results when using this gene marker for stock management. We found no evidence of departure from the expected 1 : 2 : 1 Mendelian ratio for any of the three genotypes during the egg stage, while both the 6 and 12°C temperature regimes in tank experiments favoured the survival of the Pan I^AA genotype. No difference in genotype survival was, however, found in a more natural mesocosm environment. Collectively, these results suggest that for the early life stages of Atlantic cod, and under the current experimental conditions, there is no strong consistent influence of Pan I genotype on survival. The results also emphasize the importance of varied experimental studies to verify the importance of environmental factors influencing genotype selection.

遗传标记广泛应用于世界各地的渔业管理。虽然选择的遗传结构和标记通常基于野生样本，但很少进行对照实验来研究标记之间对性状的影响可能存在的差异。在这里，我们通过在一定温度范围内进行的一系列体外杂交，检查了广泛用于大西洋鳕鱼管理的双等位基因泛素素（Pan I）。有人提出，该基因或另一个紧密连锁的基因可能处于强趋异选择之下。解决这个问题对于解释使用该基因标记进行库存管理时的结果至关重要。我们没有发现任何证据表明三种基因型在卵阶段的预期孟德尔比例为 1:2:1，而水箱实验中 6 和 12°C 的温度条件都有利于 Pan I ^AA基因型的存活。然而，在更自然的中生态环境中，基因型存活率没有差异。总的来说，这些结果表明，对于大西洋鳕鱼的早期生命阶段，在当前的实验条件下，Pan I 基因型对生存没有强烈的一致影响。结果还强调了各种实验研究的重要性，以验证环境因素影响基因型选择的重要性。