Motivation: Klebsiella species are increasingly multidrug resistant pathogens affecting human and animal health and are widely distributed in the environment. Among these, the Klebsiella pneumoniae species complex (KpSC), which includes seven phylogroups, is an important cause of community and hospital infections. In addition, the Klebsiella oxytoca species complex (KoSC) also causes hospital infections and antibiotic-associated haemorrhagic colitis. The unsuitability of widely used clinical microbiology methods to distinguish species within each of these species complexes leads to high rates of misidentifications that are masking the true clinical significance and potential epidemiological specificities of individual species. Results: We developed a web-based tool, Klebsiella MALDI TypeR, a platform-independent and user-friendly application that enables uploading raw data from MALDI-TOF mass spectrometer to identify Klebsiella isolates at the species complex and phylogroup levels. The tool is based on a database of previously identified biomarkers that are specific for either the species complex, individual phylogroups, or related phylogroups, and is available at https://maldityper.pasteur.fr.

中文翻译：

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Klebsiella MALDI TypeR：基于网络的基于MALDI-TOF质谱法鉴定克雷伯菌的工具

动机：克雷伯菌属（Klebsiella）是越来越多的耐多药病原体，影响人类和动物的健康，并广泛分布于环境中。其中，肺炎克雷伯菌（Klebsiella pneumoniae）物种复合体（KpSC）包括七个菌群，是社区和医院感染的重要原因。此外，产酸克雷伯菌种复合物（KoSC）还会引起医院感染和抗生素相关的出血性结肠炎。广泛使用的临床微生物学方法不适用于区分每种物种复合物中的物种，导致误识率很高，掩盖了单个物种的真正临床意义和潜在的流行病学特异性。结果：我们开发了基于网络的工具Klebsiella MALDI TypeR，一个独立于平台且易于使用的应用程序，可从MALDI-TOF质谱仪上载原始数据，从而在物种复合体和系统群水平上鉴定克雷伯菌。该工具基于先前识别的生物标志物的数据库，这些标志物对物种复合体，单个系统群或相关系统群具有特异性，可从https://maldityper.pasteur.fr获得。