Abstract

Various surface bioactivation technology has been confirmed to improve the osteogenic ability of porous titanium (pTi) implants effectively. In this study, a three-layered composite coating, i.e. outer layer of hydroxyapatite (HA), middle layer of loose titanium dioxide (L-TiO₂) and inner layer of dense TiO₂ (D-TiO₂), was fabricated on pTi by a combined processing procedure of pickling, alkali heat (AH), anodic oxidation (AO), electrochemical deposition (ED) and hydrothermal treatment (HT). After soaking in simulated body fluid for 48 h, the surface of the AHAOEDHT-treated pTi was completely covered by a homogeneous apatite layer. Using MC3T3-E1 pro-osteoblasts as cell model, the cell culture revealed that both the pTi without surface treatment and the AHAOEDHT sample could support the attachment, growth and proliferation of the cells. Compared to the pTi sample, the AHAOEDHT one induced higher expressions of osteogenesis-related genes in the cells, including alkaline phosphatase, Type I collagen, osteopontin, osteoclast inhibitor, osteocalcin and zinc finger structure transcription factor. As thus, besides the good corrosion resistance, the HA/L-TiO₂/D-TiO₂-coated pTi had good osteogenic activity, showing good potential in practical application for bone defect repair.

中文翻译：

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多孔钛上HA / L-TiO2 / D-TiO2复合涂层的优化制备及其对成骨细胞行为的影响

摘要

已经证实各种表面生物活化技术可以有效提高多孔钛（pTi）植入物的成骨能力。在这项研究中，三层复合涂层，即羟基磷灰石（HA）的外层，疏松的二氧化钛（L-TiO ₂）的中层和致密TiO _2的内层（D-TiO _2））是通过酸洗，碱热（AH），阳极氧化（AO），电化学沉积（ED）和水热处理（HT）的组合加工程序在pTi上制造的。在模拟体液中浸泡48小时后，AHAOEDHT处理过的pTi表面被均匀的磷灰石层完全覆盖。使用MC3T3-E1前成骨细胞作为细胞模型，细胞培养表明未经表面处理的pTi和AHAOEDHT样品均可支持细胞的附着，生长和增殖。与pTi样品相比，AHAOEDHT诱导了细胞中与成骨相关基因的更高表达，包括碱性磷酸酶，I型胶原，骨桥蛋白，破骨细胞抑制剂，骨钙素和锌指结构转录因子。因此，除了良好的耐腐蚀性外，HA / L-TiO₂ / D-TiO ₂包覆的pTi具有良好的成骨活性，在骨缺损修复的实际应用中显示出良好的潜力。