Abstract

The repair of infective bone defects is a great challenge in clinical work. It is of vital importance to develop a kind of bone scaffold with good osteogenic properties and long-term antibacterial activity for local anti-infection and bone regeneration. A porous mineralized collagen (MC) scaffold containing poly(d,l-lactide-co-glycolic acid) (PLGA) microspheres loaded with two antibacterial synthetic peptides, Pac-525 or KSL-W was developed and characterized via scanning electron microscopy (SEM), porosity measurement, swelling and mechanical tests. The results showed that the MC scaffold embedded with smooth and compact PLGA microspheres had a positive effect on cell growth and also had antibacterial properties. Through toxicity analysis, cell morphology and proliferation analysis and alkaline phosphatase evaluation, the antibacterial scaffolds showed excellent biocompatibility and osteogenic activity. The antibacterial property evaluated with Staphylococcus aureus and Escherichia coli suggested that the sustained release of Pac-525 or KSL-W from the scaffolds could inhibit the bacterial growth aforementioned in the long term. Our results suggest that the antimicrobial peptides-loaded MC bone scaffold has good antibacterial and osteogenic activities, thus providing a great promise for the treatment of infective bone defects.

中文翻译：

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载有抗菌肽的矿化胶原蛋白骨支架用于感染性骨缺损修复的开发

摘要

感染性骨缺损的修复是临床工作中的巨大挑战。开发一种具有良好成骨性和长期抗菌活性的骨支架对于局部抗感染和骨再生至关重要。含有聚（d，l开发了载有两种抗菌合成肽Pac-525或KSL-W的β-丙交酯-乙醇酸（PLGA）微球，并通过扫描电子显微镜（SEM），孔隙率测量，溶胀和机械测试对其进行了表征。结果表明，包埋有光滑致密的PLGA微球的MC支架对细胞生长具有积极作用，并且具有抗菌性能。通过毒性分析，细胞形态和增殖分析以及碱性磷酸酶评估，该抗菌支架表现出优异的生物相容性和成骨活性。金黄色葡萄球菌和大肠杆菌评价的抗菌性能提示从支架上持续释放Pac-525或KSL-W可以长期抑制上述细菌的生长。我们的结果表明，载有抗菌肽的MC骨支架具有良好的抗菌和成骨活性，因此为治疗感染性骨缺损提供了广阔的前景。