The aim of this study was to investigate the effects of Cactus (Opuntia spp) levels in total mixed ration silages based on Cactus and Gliricidia (Gliricidia sepium (Jacq.) Steud) on the fermentation profile, microbial populations, aerobic stability and taxonomic diversity. The completely randomized design was used in a 4 × 4 factorial design with four replications, being four rations with different levels of Cactus (15, 30, 45, 60% based on the dry matter) and four opening periods (0, 15, 30 and 60 days of fermentation). An interaction effect (P < 0.050) was observed among the diets and opening times for mould and yeast populations. An interaction effect for the levels of acetic acid was observed, where the diets 15, 30, 45 and 60% showed higher values at 60 days (0.44, 0.41, 0.35 and 0.40 g/kg DM, respectively). A significant difference was observed for the richness and diversity index (Chao1 and Shannon). The most abundant bacterial phyla were Proteobacteria and Firmicutes and the genera Lactobacillus and Weissella. Cactus can be added in total mixed ration silages up to the level of 60% in a way that it positively affects the qualitative indicators of the silages, modulating the taxonomic communities and allowing the predominance of important groups for preservation of the ensiled mass.

中文翻译：

---

基于仙人掌和格利亚草的全混合日粮青贮饲料的发酵概况、微生物种群、分类多样性和好氧稳定性

本研究的目的是调查仙人掌的影响（仙人掌spp) 水平在基于仙人掌和 Gliricidia (枸杞(Jacq.) Steud) 关于发酵概况、微生物种群、需氧稳定性和分类多样性。完全随机设计用于 4 × 4 因子设计，具有 4 次重复，即具有不同水平仙人掌的四种口粮（基于干物质的 15、30、45、60%）和四个开放期（0、15、30和 60 天的发酵）。交互作用（磷 <0.050）在霉菌和酵母种群的饮食和开放时间中观察到。观察到乙酸水平的相互作用效应，其中 15%、30%、45% 和 60% 的日粮在 60 天时显示出更高的值（分别为 0.44、0.41、0.35 和 0.40 g/kg DM）。观察到丰富度和多样性指数（Chao1和Shannon）存在显着差异。最丰富的细菌门是变形菌门和厚壁菌门以及乳杆菌属和魏斯氏菌属。仙人掌可以添加到总混合日粮青贮饲料的 60% 水平，从而对青贮饲料的质量指标产生积极影响，调节分类群落并允许重要群体占优势以保护青贮质量。