Both molecular analyses and culture-dependent isolation were combined to investigate the diversity of sulfate-reducing prokaryotes and explore their role in sulfides production in full-scale anaerobic digesters (Marrakech, Morocco). At global scale, using 16S rRNA gene sequencing, Proteobacteria, Bacteroidetes, Firmicutes, Actinobacteria, Synergistetes, and Euryarchaeota were the most dominant phyla. The abundance of Archaea (3.1–5.7%) was linked with temperature. The mcrA gene ranged from 2.18 × 10⁵ to 1.47 × 10⁷ gene copies.g⁻¹ of sludge. The sulfate-reducing prokaryotes, representing 5% of total sequences, involved in sulfides production were Peptococcaceae, Syntrophaceae, Desulfobulbaceae, Desulfovibrionaceae, Syntrophobacteraceae, Desulfurellaceae, and Desulfobacteraceae. Furthermore, dsrB gene ranged from 2.18 × 10⁵ to 1.92 × 10⁷ gene copies.g⁻¹ of sludge. The results revealed that exploration of diversity and function of sulfate-reducing bacteria may play a key role in decreasing sulfide production, an undesirable by-product, during anaerobic digestion.

中文翻译：

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来自废水处理厂的市政厌氧污水污泥消化池中的微生物群落和减少硫酸盐的微生物的丰度和多样性（摩洛哥马拉喀什）

结合分子分析和依赖于培养物的分离，研究了硫酸盐还原原核生物的多样性，并探讨了它们在大规模厌氧消化池（摩洛哥马拉喀什）中硫化物生产中的作用。在全球范围内，使用16S rRNA基因测序，变形杆菌，拟杆菌，Firmicutes，放线菌，协同菌和Euryarchaeota是最主要的种群。古细菌的丰度（3.1-5.7％）与温度有关。所述MCRA基因范围从2.18×10 ⁵至1.47×10 ⁷基因copies.g ^-1污泥。硫酸盐还原原核生物，占总序列的5％，参与硫化物产量分别Peptococcaceae，Syntrophaceae，Desulfobulbaceae，Desulfovibrionaceae，Syntrophobacteraceae，Desulfurellaceae，和Desulfobacteraceae。此外，dsrB基因的范围为2.18×10 ⁵至1.92×10 ^7个基因拷贝·g ^-1污泥。结果表明，探索减少硫酸盐的细菌的多样性和功能可能在减少厌氧消化过程中减少不希望产生的副产物硫化物的产生中起关键作用。