Drought stress limits plant growth and productivity. It triggers many responses by inducing changes in plant morphology and physiology. KDML105 rice is a key rice variety in Thailand and is normally grown in the northeastern part of the country. The chromosome segment substitution lines (CSSLs) were developed by transferring putative drought tolerance loci (QTLs) on chromosome 1, 3, 4, 8, or 9 into the KDML105 rice genome. CSSL104 is a drought-tolerant line with higher net photosynthesis and leaf water potential than KDML105 rice. The analysis of CSSL104 gene regulation identified the loci associated with these traits via gene co-expression network analysis. Most of the predicted genes are involved in the photosynthesis process. These genes are also conserved in Arabidopsis thaliana. Seven genes encoding chloroplast proteins were selected for further analysis through characterization of Arabidopsis tagged mutants. The response of these mutants to drought stress was analyzed daily for seven days after treatment by scoring green tissue areas via the PlantScreen™ XYZ system. Mutation of these genes affected green areas of the plant and stability index under drought stress, suggesting their involvement in drought tolerance.

中文翻译：

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基于水稻染色体替代系的基因组比较和共表达网络分析的耐旱基因鉴定

干旱胁迫限制了植物的生长和生产力。它通过诱导植物形态和生理的变化来触发许多反应。KDML105 水稻是泰国的主要水稻品种，通常生长在该国的东北部。通过将染色体 1、3、4、8 或 9 上推定的耐旱基因座 (QTL) 转移到 KDML105 水稻基因组中，开发了染色体片段替代系 (CSSL)。CSSL104 是一种耐旱品系，比 KDML105 水稻具有更高的净光合作用和叶水势。CSSL104 基因调控分析通过基因共表达网络分析确定了与这些性状相关的基因座。大多数预测基因都参与了光合作用过程。这些基因在拟南芥中也是保守的。通过对拟南芥标记突变体的表征，选择了七个编码叶绿体蛋白的基因进行进一步分析。在处理后的 7 天内，通过 PlantScreen™ XYZ 系统对绿色组织区域进行评分，每天分析这些突变体对干旱胁迫的反应。这些基因的突变影响了植物在干旱胁迫下的绿地面积和稳定性指数，表明它们参与了耐旱性。