Salvia sclarea essential oil is used as an aromatic therapy for dysmenorrhea. Sclareol—one of the natural products isolated from S. sclarea—displays anti-inflammatory and antioxidant activities; however, researchers have not yet evaluated the mechanism related to the pain-relieving effect of sclareol. In the present study, we aimed to investigate the potential effect of sclareol in ex vivo and in vivo dysmenorrhea models, as well as its possible mechanism. In the ex vivo study of uterine tissue from Sprague Dawley (SD) rats, the uterine contraction amplitude was observed and recorded. In the in vivo study, we measured the uterine contraction pressure of SD rats and performed writhing tests on mice. The uterine tissues from the writhing test subjects were collected and analyzed by Western blot. The results demonstrated that sclareol inhibited prostaglandin (PG) F_2α-, oxytocin-, acetylcholine-, carbachol-, KCl-, and Bay K 8644-induced uterine contraction and possessed an analgesic effect in the writhing test. Sclareol affects the Ca²⁺ level and regulates oxytocin receptor (OTR), myosin light chain kinase (MLCK), extracellular signal-regulated kinase, p-p38, cyclooxygenase-2 (COX-2), and phospho-myosin light chain 20 (p-MLC20) protein expression. Integrating these results, we suggest that sclareol is a potential alternative supplement for dysmenorrhea.

中文翻译：

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丹参精油提取物及其抗氧化植物化学香紫苏醇通过抑制Ca2 + –MLCK–MLC20信号级联反应抑制催产素诱导的子宫过度收缩痛经模型：一项体内和体外研究

丹参中的精油被用作痛经的芳香疗法。香紫苏醇-一种从S. sclarea中分离出来的天然产物-显示抗炎和抗氧化活性；然而，研究人员尚未评估与香紫苏醇止痛作用有关的机制。在本研究中，我们旨在研究香紫苏醇在离体和体内痛经模型中的潜在作用及其可能的机制。在来自Sprague Dawley（SD）大鼠子宫组织的离体研究中，观察并记录了子宫收缩幅度。在体内研究中，我们测量了SD大鼠的子宫收缩压力，并对小鼠进行了扭体试验。收集来自扭绞的受试者的子宫组织，并通过蛋白质印迹法进行分析。结果表明，香紫苏醇抑制前列腺素（PG）_F2α-，催产素-，乙酰胆碱-，卡巴胆碱-，KCl-和Bay K 8644引起的子宫收缩，在扭体试验中具有镇痛作用。香紫苏会影响Ca ²⁺水平并调节催产素受体（OTR），肌球蛋白轻链激酶（MLCK），细胞外信号调节激酶，p-p38，环氧合酶2（COX-2）和磷酸肌球蛋白轻链20（ p-MLC20）蛋白表达。综合这些结果，我们建议香紫苏醇是痛经的潜在替代补充剂。