ABSTRACT Pomegranate (Punica granatum) is one of the most popular fruits which is rich in nutrients and varied in cultivars including Hybrid, Wonderful (a sweet and delicious fruit with big size), and Seedless (sweet taste and very juicy). In this research, micropropagation of hybrid pomegranate cultivar (Wonderful and Seedless) was investigated. For the primary culture, the lateral and apical buds of pomegranate plant were used, and the plant materials were disinfected by Mercuric Chloride in the presence of Ascorbic Acid. Then disinfected explants cultivated to MS culture medium supplemented by 0.2 mg l−1 BAP and IBA 0.01 mgl −1 as an initial culture medium. For elongation and proliferation, BAP at five levels of 0, 0.2, 0.8, 1.4, and 2 mgl −1, and IBA at two levels of 0 and 0.01 mgl−1 were used in woody plant medium (WPM). To determine the most suitable rooting medium, IBA was used at concentrations of 0, 0.5, and 1 mgl−1 in different culture media containg MS, WPM, and DKW. Different soil compositions were also used for the adaptation of plantlets. The results showed that the best concentration for Mercuric Chloride disinfectant was 0.1% and all the disinfection treatment times were appropriate. Moreover, woody plant medium with 1.4 mg l−1 of BAP without IBA was the best culture medium for proliferation. In rooting stage, using woody plant medium was detected as the most suitable too. In the adaptation stage, using Vermicompost, Perlite, and Cocopeat (1: 1: 1) was observed to be the best treatment.

中文翻译：

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石榴体外繁殖不同培养基和激素浓度的比较

摘要 石榴 (Punica granatum) 是最受欢迎的水果之一，其营养丰富，品种繁多，包括 Hybrid、Wonderful（一种甜美可口的大果实）和 Seedless（味甜多汁）。在这项研究中，研究了杂交石榴品种（Wonderful and Seedless）的微繁殖。对于初级培养，使用石榴植物的侧芽和顶芽，并在抗坏血酸存在下用氯化汞对植物材料进行消毒。然后将消毒的外植体培养到 MS 培养基中，辅以 0.2 mg l-1 BAP 和 IBA 0.01 mgl -1 作为初始培养基。对于伸长和增殖，在木本植物培养基 (WPM) 中使用 0、0.2、0.8、1.4 和 2 mgl -1 五个水平的 BAP 和 0 和 0.01 mgl-1 两个水平的 IBA。为了确定最合适的生根培养基，IBA 在含有 MS、WPM 和 DKW 的不同培养基中以 0、0.5 和 1 mgl-1 的浓度使用。不同的土壤成分也用于适应小苗。结果表明，氯化汞消毒剂的最佳浓度为0.1%，所有消毒处理次数均适宜。此外，含有 1.4 mg l-1 BAP 且不含 IBA 的木本植物培养基是最好的增殖培养基。在生根阶段，检测到使用木本植物培养基也是最合适的。在适应阶段，观察到使用蚯蚓粪、珍珠岩和椰壳（1:1:1）是最好的处理。不同的土壤成分也用于适应小苗。结果表明，氯化汞消毒剂的最佳浓度为0.1%，所有消毒处理次数均适宜。此外，含有 1.4 mg l-1 BAP 且不含 IBA 的木本植物培养基是最好的增殖培养基。在生根阶段，检测到使用木本植物培养基也是最合适的。在适应阶段，观察到使用蚯蚓粪、珍珠岩和椰壳（1:1:1）是最好的处理。不同的土壤成分也用于适应小苗。结果表明，氯化汞消毒剂的最佳浓度为0.1%，各消毒处理次数适宜。此外，含有 1.4 mg l-1 BAP 且不含 IBA 的木本植物培养基是最好的增殖培养基。在生根阶段，检测到使用木本植物培养基也是最合适的。在适应阶段，观察到使用蚯蚓粪、珍珠岩和椰壳（1:1:1）是最好的处理。使用木本植物培养基也被检测为最合适的。在适应阶段，观察到使用蚯蚓粪、珍珠岩和椰壳（1:1:1）是最好的处理。使用木本植物培养基也被检测为最合适的。在适应阶段，观察到使用蚯蚓粪、珍珠岩和椰壳（1:1:1）是最好的处理。