ABSTRACT The study was conducted to investigate the effect of different levels of protein supplements on fertility and assess the fertility gene (BMP15 and GDP9) in sheep of Bangladesh. Thirty six ewes fed two protein level (Treatment 1, T1 and treatment 2, T2) and no protein supplement ration (control, T0). Polymerase chain reactions (PCR) were performed from blood cell DNA following standard protocols. PCR products were sequenced using Sanger sequencing and MEGA6 software were used for molecular analysis. The conception rate of ewes was significantly higher (P < 0.05) in the treated group than the control. Gestation period and lambing interval were significantly higher (worse) in control (154.00 ± 0.57 and 186.00 ± 1.24 days) than treated groups. Birth weight of lamb ranged from 1.07 to 1.65 kg and higher birth weight was found in T2 than other groups. Sequence alignment of BMP15 and GDF9 gene in indigenous sheep showed 100% homology with other sheep breed. As no mutation was observed and apparently these genes have no effect on ewe fertility. However, nutritional supplements had a positive impact on reproductive performance. The phylogenetic inferences have close relations of Bangladeshi sheep with other breed elsewhere.

中文翻译：

---

蛋白质补充剂对孟加拉国本土绵羊生育力的影响和生育力基因（GDF9 和 BMP15）的评估

摘要 本研究旨在研究不同水平蛋白质补充剂对孟加拉绵羊生育力的影响，并评估其生育力基因（BMP15 和 GDP9）。三十六只母羊饲喂两种蛋白质水平（处理 1，T1 和处理 2，T2）和无蛋白质补充日粮（对照，T0）。按照标准方案从血细胞 DNA 进行聚合酶链反应 (PCR)。PCR产物用Sanger测序法测序，MEGA6软件用于分子分析。治疗组母羊受胎率显着高于对照组（P < 0.05）。对照组（154.00 ± 0.57 和 186.00 ± 1.24 天）的妊娠期和产羔间隔显着高于（更差）治疗组。羔羊的出生体重在 1.07 到 1 之间。与其他组相比，T2 组的出生体重为 65 kg 和更高。本地绵羊BMP15和GDF9基因的序列比对与其他绵羊品种具有100%的同源性。由于没有观察到突变，显然这些基因对母羊的生育能力没有影响。然而，营养补充剂对繁殖性能有积极影响。系统发育推断孟加拉绵羊与其他地方的其他品种关系密切。