Abstract

Fulvic acid (FA) is a natural mineral medicine with a long medical history in folk. However, the active chemicals of FA remain unknown due to its diversity of sources and the complexity of compositions, which have become a bottleneck in quality control and medicinal development. Based on the traditional effect on angiogenesis, FAs from eight different coal sources were prepared and their active fractions were investigated by the CAM model, resulting that most of acetonitrile dissolved parts of these FAs (DFAs) produced angiogenesis effects. Through chemical analysis on DFAs by GC-FID/MS, six shared organic acids with low molecular weights were identified and quantified, which showed the promoting effects on capillary areas, VEGF, b-FGF, and Ang-1 at different degrees. The PCA analysis showed that the five shared organic acids with high recognition are the active chemicals in different sources of FAs which may be responsible for the angiogenesis effects.

摘要

富里酸（FA）是民间医学历史悠久的天然矿物药。然而，由于其来源的多样性和成分的复杂性，FA的活性化学物质仍然未知，这已成为质量控制和药物开发的瓶颈。基于传统对血管生成的影响，制备了来自 8 种不同煤源的 FAs，并通过 CAM 模型研究了它们的活性部分，结果这些 FAs (DFA) 的大部分乙腈溶解部分产生了血管生成作用。通过GC-FID/MS对DFAs的化学分析，鉴定并定量了6种低分子量的共有有机酸，显示出对毛细血管面积、VEGF、b-FGF、Ang-1有不同程度的促进作用。