ABSTRACT

Cyanobacteria synthesize many secondary metabolites of various chemical structures and biological activity and release them into the aquatic environment. Two new oligopeptides belonging to anabaenopeptins and cyanopeptolins were isolated from an enrichment culture dominated by Woronichinia naegeliana (Unger) Elenkin. The application of mass spectrometry and nuclear magnetic resonance spectroscopy allowed determination of their structure and identification of these compounds as cyanopeptolin 1081 and anabaenopeptin 899 with a molecular formula C₅₂H₇₅N₉O₁₆ and C₄₈H₆₅N₇O₁₀, respectively. The preliminary evaluation of their toxicity was conducted via tests based on crustacean Thamnocephalus platyurus. Although these peptides did not exert acute toxic effects at the concentration up to 40 μg ml^–1, deterioration of the physiological state of the studied crustaceans, indicated by reduced mobility, was observed. Considering the limited knowledge of cyanopeptide presence in the aquatic environment, it is important to identify and characterize these compounds.

摘要

蓝藻合成许多具有各种化学结构和生物活性的次级代谢产物，并将它们释放到水生环境中。从以Woronichinia naegeliana (Unger) Elenkin为主的富集培养物中分离出属于 anabaenopeptins 和 cyanopeptolins 的两种新寡肽。质谱和核磁共振波谱的应用允许确定它们的结构并鉴定这些化合物为具有分子式 C ₅₂ H ₇₅ N ₉ O ₁₆和 C ₄₈ H ₆₅ N ₇ O _{10 的}cyanopeptolin 1081 和 anabaenopeptin 899， 分别。其毒性的初步评估是通过基于甲壳动物Thamnocephalus platyurus 的试验进行的。尽管这些肽在高达 40 μg ml ^–1的浓度下不会产生急性毒性作用，但观察到所研究的甲壳类动物的生理状态恶化，表现为流动性降低。考虑到对水生环境中存在的氰肽的了解有限，识别和表征这些化合物非常重要。