Abstract

This review briefly describes the principles, methods, and applications of electronic circular dichroism (ECD) and Raman optical activity (ROA) in practical cases. Researchers have always been interested in determining the absolute configuration of natural products. Due to the characteristics of high sensitivity and simple sample requirements, ECD is widely used in the configuration of rigid systems and flexible systems. Besides, the shortcomings in protein structure and the application of visual analysis of twisted bilayer graphene structure spectra will be explained. In recent years, the combination of ECD and other methods has significantly improved time-consuming and accuracy.With the development of spectrometers, the characteristics of low ROA scattering intensity have also been improved. The review summarized several technologies of surface-enhanced ROA, as well as new technologies related to ROA measurement. The uniqueness of ROA is mainly reflected in the biological structure. It analyzes the application in protein, nucleic acid and carbohydrate. The article uses examples to introduce a new visualization method of this experimental group combined with ECD and ROA spectroscopy to analyze the chiral structure of different molecules. The anisotropy on the dipole moment of the chromophore under a specific laser will affect the spectrum, and there is a relationship between the anisotropy and the chiral specific structure. In a broad sense, this theoretical method is a general analysis of the chirality of traditional molecules, clusters, and crystals method. Due to their early development, ECD and ROA have always been powerful "probing needles" for structure determination through chiral interactions.

摘要

本综述简要介绍了电子圆二色性 (ECD) 和拉曼光学活性 (ROA) 在实际案例中的原理、方法和应用。研究人员一直对确定天然产物的绝对构型很感兴趣。由于具有灵敏度高、样品要求简单的特点，ECD广泛用于刚性系统和柔性系统的配置。此外，将解释蛋白质结构的缺点和扭曲双层石墨烯结构光谱的视觉分析的应用。近年来，ECD与其他方法的结合显着提高了耗时性和准确性。随着光谱仪的发展，低ROA散射强度的特性也得到了改善。该综述总结了几种表面增强 ROA 技术，以及与 ROA 测量相关的新技术。ROA的独特性主要体现在生物结构上。分析了在蛋白质、核酸和碳水化合物中的应用。文章以实例介绍了本实验组结合ECD和ROA光谱分析不同分子手性结构的一种新的可视化方法。特定激光下发色团偶极矩的各向异性会影响光谱，各向异性与手性特定结构之间存在关系。从广义上讲，这种理论方法是对传统分子、簇、晶体方法手性的一般分析。由于早期发展，ECD和ROA一直很强大”