Licorice extract suppresses adipogenesis through regulation of mitotic clonal expansion and adenosine monophosphate‐activated protein kinase in 3T3‐L1 cells,Journal of Food Biochemistry

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Licorice extract suppresses adipogenesis through regulation of mitotic clonal expansion and adenosine monophosphate‐activated protein kinase in 3T3‐L1 cells
Journal of Food Biochemistry ( IF 3.5 ) Pub Date : 2020-10-13 , DOI: 10.1111/jfbc.13528
Mun-Hoe Lee ₁ , Hyeong-Min Kim ₁ , Hee-Chul Chung ₁ , Jin-Hee Lee ₁

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Licorice, the root of Glycyrrhiza glabra, has been observed to possess an anti‐obesity effect. Previous research has suggested that licorice acetone extract (LE) has an influence on mitotic clonal expansion (MCE) and adenosine monophosphate‐activated protein kinase (AMPK), which play a key role in regulating adipogenesis. This study sought further insight into the molecular mechanism of LE’s anti‐obesity effect using 3T3‐L1 adipocytes in vitro. LE inhibited 3T3‐L1 adipogenesis, and the inhibitory effect of LE on adipogenesis was most significant in the early stage of adipogenic differentiation. LE inhibited the protein expression of cyclins and cyclin‐dependent kinases in the MCE stage and arrested cells in the G1 phase of the cell cycle. Furthermore, it activated AMPK via phosphorylation. Moreover, the expression levels of lipid metabolism‐related genes were regulated by LE. These findings suggest the anti‐obesity effect of LE via MCE and AMPK regulation.

中文翻译：

甘草提取物通过调节3T3–L1细胞中的有丝分裂克隆扩增和腺苷单磷酸激活的蛋白激酶来抑制脂肪形成

甘草的根，甘草被观察到具有抗肥胖作用。先前的研究表明，甘草丙酮提取物（LE）对有丝分裂克隆扩增（MCE）和单磷酸腺苷活化蛋白激酶（AMPK）有影响，它们在调节脂肪形成中起关键作用。这项研究寻求进一步了解使用3T3-L1脂肪细胞体外LE抗肥胖作用的分子机制。LE抑制3T3-L1的脂肪形成，在成脂分化的早期，LE对脂肪形成的抑制作用最为明显。LE在MCE期抑制细胞周期蛋白和细胞周期蛋白依赖性激酶的蛋白表达，并在细胞周期的G1期抑制细胞。此外，它通过磷酸化激活了AMPK。此外，脂质代谢相关基因的表达水平受LE调节。

更新日期：2020-10-13

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