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Benzo[a]pyrene exposure and overcrowding stress impacts anxiety‐like behavior and impairs learning and memory in adult zebrafish, Danio rerio
Environmental Toxicology ( IF 4.4 ) Pub Date : 2020-10-14 , DOI: 10.1002/tox.23041 Sai Aparna 1 , Manorama Patri 1
Environmental Toxicology ( IF 4.4 ) Pub Date : 2020-10-14 , DOI: 10.1002/tox.23041 Sai Aparna 1 , Manorama Patri 1
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Benzo[a]pyrene (B[a]P), a prototype of polycyclic aromatic hydrocarbons and ubiquitous environmental pollutant, alters neurobehavioral responses in aquatic organisms like zebrafish. Increasing organic load on water bodies causes population explosion leading to overcrowding (OC) stress. The effect of OC stress on neurobehavioral alterations remains unclear. The objective of our study is to elucidate the impact of OC stress on behavioral alterations and neurodegenerative phenotypes on exposure to B[a]P in zebrafish. We demonstrate the effects of OC stress (12 fish/L) on acute waterborne exposure to B[a]P (0.2 mg L-1 ) in adult wild zebrafish. Anxiety-like behavior, learning, and memory impairment were assayed by novel tank diving test, light/dark preference test, and T-maze test. Oxidative stress bio-markers were assayed along with histopathological changes in zebrafish brain. OC stress significantly impaired the learning ability and mood behavior by increasing the number of transition and time spent in the alter zones. Increased lipid peroxidation and protein carbonyl formation with significant decreased catalase activity and reduced glutathione level showed oxidative stress on exposure to OC stress and B[a]P. Pyknotic neuronal counts dramatically increased in periventricular grey zone of optic tectum brain region of zebrafish. Our findings showed that OC stress modulates the B[a]P-induced behavioral alterations causing learning and memory deficiency with histopathological changes in adult zebrafish brain. OC stress may act as an early risk factor for the eventual development of cognitive impairments and B[a]P exposure plays a key role in mediating both the facilitating and impairing actions of OC stress in memory processes.
中文翻译：
苯并[a]芘暴露和过度拥挤的压力会影响成年斑马鱼的焦虑样行为并损害学习和记忆，Danio rerio
苯并[a]芘 (B[a]P) 是多环芳烃和无处不在的环境污染物的原型，它改变了斑马鱼等水生生物的神经行为反应。水体有机负荷的增加导致人口爆炸，导致过度拥挤 (OC) 压力。OC 应激对神经行为改变的影响尚不清楚。我们研究的目的是阐明 OC 压力对斑马鱼行为改变和神经退行性表型的影响。我们证明了 OC 应激（12 条鱼/L）对成年野生斑马鱼中 B[a]P（0.2 mg L-1）急性水源性暴露的影响。焦虑样行为、学习和记忆障碍通过新型坦克潜水测试、光/暗偏好测试和 T 迷宫测试进行测定。氧化应激生物标志物与斑马鱼脑的组织病理学变化一起进行了测定。OC 压力通过增加转换区的数量和花费的时间，显着削弱了学习能力和情绪行为。脂质过氧化和蛋白质羰基形成增加，过氧化氢酶活性显着降低，谷胱甘肽水平降低，表明暴露于 OC 应激和 B[a]P 时出现氧化应激。斑马鱼视顶盖脑区脑室周围灰区的固缩神经元计数显着增加。我们的研究结果表明，OC 应激调节 B[a]P 诱导的行为改变，导致学习和记忆缺陷以及成年斑马鱼大脑的组织病理学变化。
更新日期：2020-10-14
中文翻译：
苯并[a]芘暴露和过度拥挤的压力会影响成年斑马鱼的焦虑样行为并损害学习和记忆，Danio rerio
苯并[a]芘 (B[a]P) 是多环芳烃和无处不在的环境污染物的原型，它改变了斑马鱼等水生生物的神经行为反应。水体有机负荷的增加导致人口爆炸，导致过度拥挤 (OC) 压力。OC 应激对神经行为改变的影响尚不清楚。我们研究的目的是阐明 OC 压力对斑马鱼行为改变和神经退行性表型的影响。我们证明了 OC 应激（12 条鱼/L）对成年野生斑马鱼中 B[a]P（0.2 mg L-1）急性水源性暴露的影响。焦虑样行为、学习和记忆障碍通过新型坦克潜水测试、光/暗偏好测试和 T 迷宫测试进行测定。氧化应激生物标志物与斑马鱼脑的组织病理学变化一起进行了测定。OC 压力通过增加转换区的数量和花费的时间，显着削弱了学习能力和情绪行为。脂质过氧化和蛋白质羰基形成增加，过氧化氢酶活性显着降低，谷胱甘肽水平降低，表明暴露于 OC 应激和 B[a]P 时出现氧化应激。斑马鱼视顶盖脑区脑室周围灰区的固缩神经元计数显着增加。我们的研究结果表明，OC 应激调节 B[a]P 诱导的行为改变，导致学习和记忆缺陷以及成年斑马鱼大脑的组织病理学变化。
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