Abstract Zebra mussels (Dreissena polymorpha) are commonly used as sentinel organisms in freshwater biomonitoring. The displacement of this species by quagga mussels (Dreissena rostriformis bugensis) has raised the issue of their discrimination after field sampling. Even if those two species exhibit specific morphological characteristics, confusion can occur. However, behind the visible phenotypes lie important physiological and metabolic differences that may affect the interpretation of biomarkers in risk assessment. In this study, we present a characterization of Cu/Zn-SODs (superoxide dismutases) in Dreissena spp. with a comparison of activity profiles from individuals originating from North America and Europe. A phylogenetic tree was constructed and dreissenid SOD sequences appear to be closely related to clam species sequences. A specific isoform at an isoelectric point value of 4.95 was observed only for quagga mussels independently of their origin, while zebra mussels exhibited several polymorphic profiles, even for a single sampling site. A comparison of transcript sequences was conducted and the observed differences were highlighted. Alignment of amino acid sequences shows a high level of identity between the two species associated with minor changes in the predicted secondary structures.

中文翻译：

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同域物种中 Cu/Zn-SOD 的表征：斑马和 Quagga 贻贝的比较

摘要 斑马贻贝 (Dreissena polymorpha) 常被用作淡水生物监测中的哨兵生物。斑斑贻贝 (Dreissena rostriformis bugensis) 对这一物种的替代引发了野外取样后对它们进行区分的问题。即使这两个物种表现出特定的形态特征，也可能发生混淆。然而，在可见表型背后隐藏着重要的生理和代谢差异，这些差异可能会影响对风险评估中生物标志物的解释。在这项研究中，我们介绍了 Dreissena spp 中 Cu/Zn-SOD（超氧化物歧化酶）的特征。与来自北美和欧洲的个人的活动概况进行比较。构建了系统发育树，dreissenid SOD 序列似乎与蛤物种序列密切相关。等电点值为 4.95 的特定同种型仅在与原产地无关的斑马贻贝中观察到，而斑马贻贝表现出多种多态性特征，即使对于单个采样点也是如此。进行了转录本序列的比较，并突出了观察到的差异。氨基酸序列的比对显示了两个物种之间的高度同一性，与预测的二级结构的微小变化相关。