Interest in periocular (eyelid and eyelashes margins) hygiene has attracted attention recently and a growing number of commercials eye cleanser and shampoos have been marketed. In the present study, a particular eye cleanser foam, Belcils® has been tested against trophozoites and cysts on the facultative pathogen Acanthamoeba. Viability was tested by the alamarBlue™ method and the foam was tested for the induction of programmed cell death in order to explore its mode of action. We found that a 1% solution of the foam eliminated both trophozoite and cyst stage of Acanthamoeba spp. After 90 min of incubation, Belcils® induced, DNA condensation, collapse in the mitochondrial membrane potential and reduction of the ATP level production in Acanthamoeba. We conclude that the foam destroys the cells by the induction of an apoptosis-like process. The current eye cleanser could be used as part of AK therapy protocol and as prevention from AK infections for contact lens users and post-ocular trauma patients.

最近，人们对眼周（眼睑和睫毛边缘）卫生的兴趣引起了人们的关注，并且越来越多的眼部清洁剂和洗发水已经上市。在本研究中，一种特殊的眼部清洁泡沫 Belcils® 已针对兼性病原体棘阿米巴的滋养体和包囊进行了测试。通过 alamarBlue™ 方法测试了活力，并测试了泡沫对程序性细胞死亡的诱导，以探索其作用模式。我们发现 1% 的泡沫溶液消除了棘阿米巴属的滋养体和包囊期。孵育 90 分钟后，Belcils® 诱导棘阿米巴DNA 浓缩、线粒体膜电位崩溃和 ATP 水平产生减少。我们得出的结论是，泡沫通过诱导细胞凋亡样过程来破坏细胞。目前的眼部清洁剂可用作 AK 治疗方案的一部分，并用于预防隐形眼镜使用者和眼外伤后患者的 AK 感染。