Abstract Plant NADH glutamate dehydrogenase (GDH) is an intriguing enzyme, since it is involved in different metabolic processes owing to its reversible (anabolic/catabolic) activity and due to the oligomeric nature of the enzyme, that gives rise to several isoforms. The complexity of GDH isoenzymes pattern and the variability of the spatial and temporal localization of the different isoforms have limited our comprehension of the physiological role of GDH in plants. Genetics, immunological, and biochemical approaches have been used until now in order to shed light on the regulatory mechanism that control GDH expression in different plant systems and environmental conditions. We describe here the validation of a simple in planta GDH activity staining procedure, providing evidence that it might be used, with different purposes, to determine GDH expression in plant organs, tissues, extracts and also heterologous systems.

中文翻译：

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如何轻松检测植物NADH-谷氨酸脱氢酶（GDH）活性？简单可靠的植物体内程序，适用于组织、提取物和异源微生物系统


摘要 植物 NADH 谷氨酸脱氢酶 (GDH) 是一种有趣的酶，由于其可逆（合成代谢/分解代谢）活性以及酶的寡聚性质，它参与不同的代谢过程，从而产生多种亚型。 GDH 同工酶模式的复杂性以及不同同工型的时空定位的可变性限制了我们对 GDH 在植物中生理作用的理解。迄今为止，人们一直在使用遗传学、免疫学和生化方法来阐明不同植物系统和环境条件下控制 GDH 表达的调控机制。我们在这里描述了简单的植物内 GDH 活性染色程序的验证，提供了证据表明它可以用于不同目的，以确定植物器官、组织、提取物和异源系统中的 GDH 表达。