The goal of this study was to develop a new method based on Oncothermia with concomitant use of the temozolomide (TMZ)-loaded magnetic nanoparticles conjugated with folic acid (TMZ/MNPs-FA) and alternative magnetic field (AMF) and evaluate its efficacy in the treatment of C6 glioma in rats. TMZ/MNPs-FA were prepared and evaluated for their size, surface charge, magnetic saturation, hemolysis and in vitro AMF-triggered release. The glioma rat models were treated with free TMZ, MNPs-FA and TMZ/MNPs-FA in the presence or absence of AMF (43 °C). The results confirmed that a combinatorial therapy consisting of AFM hyperthermia and thermosensitive TMZ/MNPs-FA could significantly suppress tumor growth, increase survival rate and promote apoptosis (P < 0.0001). Therefore, this treatment strategy may be a powerful modality for treatment of cancer, as the thermal and mechanical effects of magnetic nanoparticles exposed to AMF can increase the therapeutic efficacy of conventional chemotherapy.

本研究的目标是开发一种基于 Oncothermia 的新方法，同时使用载有替莫唑胺 (TMZ) 的磁性纳米粒子与叶酸 (TMZ/MNPs-FA) 和交变磁场 (AMF) 结合，并评估其在治疗中的功效。治疗大鼠 C6 胶质瘤。制备并评估了 TMZ/MNPs-FA 的大小、表面电荷、磁饱和度、溶血和体外 AMF 触发的释放。在存在或不存在 AMF (43 °C) 的情况下，用游离 TMZ、MNPs-FA 和 TMZ/MNPs-FA 处理神经胶质瘤大鼠模型。结果证实，AFM 热疗和热敏 TMZ/MNPs-FA 联合治疗可显着抑制肿瘤生长，提高存活率，促进细胞凋亡（P < 0.0001)。因此，这种治疗策略可能是一种强大的癌症治疗方式，因为暴露于 AMF 的磁性纳米粒子的热和机械效应可以提高常规化疗的治疗效果。