Eggshell membrane, an eco-compatible, safe and cheap by-product was employed as carrier for the laccase from Trametes versicolor immobilization. In order to evaluate the best protocol to apply for the syringic acid degradation, two different types of laccase loading on eggshell membrane were used by incubation in solution or by enzyme-dropping. Chemicals (covalent) and physicals (adsorption) immobilizations were tested for both procedure using native or periodate-oxidized laccase. It is shown that immobilization of periodate-oxidized laccase on NiCl₂-pretreated eggshell membrane was the best method for the first procedure (immobilized activity 1300 U/Kg, a residual activity of 30 % for 6 reuse). For the enzyme-dropping protocol a covalent method with the bifunctional cross linker (glutaraldehyde) was the best method (immobilized activity 3500 U/Kg, a residual activity of 45 % for 6 reuse).

蛋壳膜是一种生态相容、安全且廉价的副产品，被用作固定花斑栓菌漆酶的载体。为了评估适用于丁香酸降解的最佳方案，通过在溶液中孵育或酶滴加，使用了两种不同类型的蛋壳膜上的漆酶负载。使用天然或高碘酸盐氧化漆酶对这两种方法的化学（共价）和物理（吸附）固定进行了测试。结果表明，高碘酸氧化漆酶在 NiCl ₂上的固定化- 预处理的蛋壳膜是第一个程序的最佳方法（固定活性 1300 U/Kg，6 次重复使用的残留活性为 30%）。对于酶滴法，使用双功能交联剂（戊二醛）的共价方法是最好的方法（固定活性 3500 U/Kg，6 次重复使用的残留活性为 45%）。