Twice-daily (BID) insulin injections are a major deterrence to owners treating dogs with diabetes mellitus (DM). The hypothesis for this study was that Protamine Zinc Recombinant Human Insulin (PZIR) is safe and efficacious as a once-daily (SID) treatment for canine DM. This was a prospective, baseline-controlled, multi-center study over 182 ± 5 d. Two hundred seventy-six client-owned dogs with naturally occurring DM (naïve or pre-treated with insulin) were enrolled in the study. Enrollment was based upon demonstration of hyperglycemia, glycosuria, and ≥1 diabetic clinical sign (polyuria (PU), polydipsia (PD), or weight loss). Insulin treatment was initiated at 0.5–1.0 IU/kg SID. An improvement in at least one lab parameter related to DM (mean BG, min BG, Fructosamine) and one clinical parameter (PU/PD, body weight) was achieved in 72% of dogs (80% of naive, 62% of pre-treated). Dogs treated SID and BID showed improvement in 71% and 74% of cases, respectively. In naïve dogs, mean and minimum BG and fructosamine were significantly decreased (P < 0.05) by d 7 and 21, respectively, and in pre-treated dogs by d 63. By d 84, PU/PD improved in 90% and 88% of dogs, respectively, and the mean successful insulin dose was 1.4 IU/kg/d. Safety parameters were measured in 276 dogs for up to 182 d; clinical hypoglycemia occurred in 8.9% of dogs. We conclude that PZIR safely and effectively improved glycemic parameters and clinical signs in naïve and pre-treated diabetic dogs. The significant percentage of dogs on SID treatment with improvement in hyperglycemia and clinical signs confirms the prolonged action of PZIR in many dogs.

每天两次 (BID) 胰岛素注射是对治疗患有糖尿病 (DM) 的狗的主人的主要威慑。这项研究的假设是，精蛋白锌重组人胰岛素 (PZIR) 作为犬 DM 的每日一次 (SID) 治疗是安全和有效的。这是一项超过 182 ± 5 天的前瞻性、基线对照、多中心研究。276 只患有自然发生 DM 的客户拥有的狗（未接受过胰岛素治疗或预先用胰岛素治疗）被纳入研究。入组是基于高血糖、糖尿和≥1 种糖尿病临床症状（多尿 (PU)、烦渴 (PD) 或体重减轻）的证明。胰岛素治疗以 0.5-1.0 IU/kg SID 开始。至少一项与 DM 相关的实验室参数（平均 BG、最小 BG、果糖胺）和一项临床参数（PU/PD、72% 的狗（80% 的幼犬，62% 的预处理）达到了体重）。接受 SID 和 BID 治疗的狗分别在 71% 和 74% 的病例中表现出改善。在幼犬中，平均和最低 BG 和果糖胺显着降低（P < 0.05）分别在第 7 天和第 21 天，在预处理的狗中，在第 63 天。到第 84 天，PU/PD 分别在 90% 和 88% 的狗中得到改善，平均成功的胰岛素剂量为 1.4 IU /公斤/天。对 276 只狗进行了长达 182 天的安全参数测量；8.9%的狗出现临床低血糖。我们得出结论，PZIR 安全有效地改善了幼稚和预处理糖尿病犬的血糖参数和临床症状。接受 SID 治疗并改善高血糖和临床症状的狗的显着百分比证实了 PZIR 在许多狗中的延长作用。