This study has investigated on two objectives which are enhancing the microalgae Chlorella sorokiniana CY-1 cell disruption mechanism using thermal Fenton reaction and performing non-catalytic transesterification by ultra-sonication method. Radicles generated by Fenton reaction are rapid, dynamic, and the reaction was assisted by thermal heating to optimize the kinetic of the radical generation by obtained the lipid recovery at 53.3% and 977.41 mg/g at 36.5 ^oC with 4 min of contact time. The conversion of lipid into fatty acid methyl esters (FAME) through rapid non-catalytic transesterification using ultrasound was also performed. The significant parameters were done by varying the ratio of methanol to lipid, duration and pulse mode of sonication, to optimize the bonding reaction with methyl ester compound to free fatty acids. The formed of hydrocarbon chains with C4, C6, and C10 at 8.20 mg/g, 0.33 mg/g, and 0.24 mg/g respectively. This is a promising method as it is marketable and can be conducted in large scale with substantial improvement on the process yield of F.A.M.E in a rapid manner.