This study has investigated on two objectives which are enhancing the microalgae Chlorella sorokiniana CY-1 cell disruption mechanism using thermal Fenton reaction and performing non-catalytic transesterification by ultra-sonication method. Radicles generated by Fenton reaction are rapid, dynamic, and the reaction was assisted by thermal heating to optimize the kinetic of the radical generation by obtained the lipid recovery at 53.3% and 977.41 mg/g at 36.5 ^oC with 4 min of contact time. The conversion of lipid into fatty acid methyl esters (FAME) through rapid non-catalytic transesterification using ultrasound was also performed. The significant parameters were done by varying the ratio of methanol to lipid, duration and pulse mode of sonication, to optimize the bonding reaction with methyl ester compound to free fatty acids. The formed of hydrocarbon chains with C4, C6, and C10 at 8.20 mg/g, 0.33 mg/g, and 0.24 mg/g respectively. This is a promising method as it is marketable and can be conducted in large scale with substantial improvement on the process yield of F.A.M.E in a rapid manner.

本研究研究了两个目标，即利用热芬顿反应增强微藻小球藻小球藻CY-1细胞破坏机制和通过超声方法进行非催化酯交换反应。Fenton反应产生的自由基快速，动态，并且通过加热加热辅助反应，通过在36.5 ^o下获得53.3％的脂质回收率和977.41 mg / g来优化自由基生成的动力学。C，接触时间为4分钟。还使用超声波通过快速非催化酯交换将脂质转化为脂肪酸甲酯（FAME）。通过改变甲醇与脂质的比例，超声的持续时间和脉冲模式来确定重要参数，以优化与甲酯化合物与游离脂肪酸的键合反应。由C4，C6和C10分别为8.20 mg / g，0.33 mg / g和0.24 mg / g的烃链形成。这是一种有前途的方法，因为它是可销售的，并且可以大规模进行，并且快速地大大改善了FAME的工艺产量。