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A post-insertion strategy for surface functionalization of bacterial and mammalian cell-derived extracellular vesicles
Biochimica et Biophysica Acta (BBA) - General Subjects ( IF 3 ) Pub Date : 2020-10-14 , DOI: 10.1016/j.bbagen.2020.129763
Linglei Jiang 1 , Joen Luirink 2 , Sander A A Kooijmans 1 , Kok P M van Kessel 3 , Wouter Jong 4 , Max van Essen 1 , Cor W Seinen 1 , Steven de Maat 1 , Olivier G de Jong 1 , Jerney F F Gitz-François 1 , Wim E Hennink 5 , Pieter Vader 6 , Raymond M Schiffelers 1
Affiliation  

Extracellular vesicles (EVs) are nanoparticles which are released by cells from all three domains of life: Archaea, Bacteria and Eukarya. They can mediate cell-cell communication by transferring cargoes such as proteins and nucleic acids between cells. EVs receive great interest in both academia and industry as they have the potential to be natural drug carriers or vaccine candidates. However, limitations to their clinical translation exist as efficient isolation, loading, labelling and surface-engineering methods are lacking. In this article, we investigate a ‘post-insertion’ approach, which is commonly used in the functionalization of liposomes in the pharmaceutical field, on two different EV types: mammalian cell-derived EVs and bacteria-derived EVs. We aimed to find an easy and flexible approach to functionalize EVs, thereby improving the labelling, isolation, and surface-engineering.



中文翻译:

细菌和哺乳动物细胞衍生的细胞外囊泡表面功能化的插入后策略

细胞外囊泡(EVs)是纳米颗粒,细胞生活中的三个域(古细菌,细菌和Eukarya)都会释放它们。它们可以通过在细胞之间转移货物(例如蛋白质和核酸)来介导细胞间的通信。电动汽车在学术界和工业界都倍受关注,因为它们有可能成为天然药物载体或候选疫苗。但是,由于缺乏有效的隔离,装载,标记和表面工程方法,因此对其临床翻译存在限制。在本文中,我们研究了“插入后”方法,该方法通常用于制药领域中脂质体的功能化,适用于两种不同的EV类型:哺乳动物细胞源的EV和细菌源的EV。我们旨在寻找一种简便灵活的方法来使电动汽车功能化,

更新日期:2020-10-15
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