This study was conducted to assess effects of different doses of pFSH on follicular recruitment, superovulatory response, ova/embryo recovery, and embryo yield in lactating ewes. Ewes (n = 24) had a superovulation treatment regimen imposed. All ewes were implanted with a progesterone intravaginal device for 9 d, and administered either 100 (G-100) or 200 (G-200) mg pFSH, proportioned into six doses administered at 12-h intervals, starting 60 h before device removal. At 7 days subsequent to progesterone device removal, there were non-surgical embryo recoveries (NSER) from ewes having three or more corpora lutea. At the time of the first pFSH injection, number of antral follicles were similar (P < 0.05) between ewes in the G-100 and G-200 group, however, there were more 3.1–4.0 mm follicles in ewes of the G-200 than G-100 group at the time of the second pFSH administration. Estrous response and CL number were less (P < 0.05) in ewes of the G-100 (66.7 % and 2.6 ± 0.7) than G-200 (91.7 % and 11.6 ± 1.2) group. There were embryo collections from 100 % and 90.9 % of ewes in the G-100 and G-200 groups, respectively (P > 0.05). Viable embryo numbers and ova/embryo recovery rate were greater (P < 0.05) in ewes of the G-200 (6.9 ± 1.1 and 67.8 %) than G-100 (1.0 ± 0.5 and 27.6 %) group. A dose of 200 mg pFSH was more effective in inducing a superovulatory response and embryo yield after NSER in ewes, however, the 100 mg dose was insufficient for these purposes.

本研究旨在评估不同剂量的 pFSH 对泌乳母羊的卵泡募集、超排卵反应、卵/胚胎恢复和胚胎产量的影响。母羊 ( n  = 24) 强加了超排卵治疗方案。所有母羊都植入孕酮阴道内装置 9 天，并给予 100 (G-100) 或 200 (G-200) 毫克 pFSH，按 12 小时间隔分配成六剂，从装置移除前 60 小时开始。在去除黄体酮装置后 7 天，有来自具有三个或更多黄体的母羊的非手术胚胎恢复 (NSER)。第一次注射 pFSH 时，窦卵泡数量相似（P < 0.05) G-100 和 G-200 组的母羊之间，然而，在第二次 pFSH 给药时，G-200 的母羊比 G-100 组有更多 3.1-4.0 毫米的卵泡。 G-100（66.7 % 和 2.6 ± 0.7）组母羊的发情反应和 CL 数低于 G-200（91.7 % 和 11.6 ± 1.2）组（P < 0.05）。G-100 和 G-200 组的母羊分别有 100% 和 90.9% 的胚胎采集（P  > 0.05）。存活胚胎数和卵子/胚胎恢复率更高（P < 0.05) 在 G-200（6.9 ± 1.1 和 67.8 %）的母羊中比 G-100（1.0 ± 0.5 和 27.6 %）组的母羊。200 毫克 pFSH 的剂量在诱导母羊 NSER 后超排卵反应和胚胎产量方面更有效，但是，100 毫克剂量不足以达到这些目的。