Aegerolysins are small secreted pore-forming proteins that are found in both prokaryotes and eukaryotes. The role of aegerolysins in sporulation, fruit body formation, and in lysis of cellular membrane is suggested in fungi. The aim of the present study was to characterize the biological function of the aegerolysin gene agl1 in the mycoparasitic fungus Trichoderma atroviride, used for biological control of plant diseases. Gene expression analysis showed higher expression of agl1 during conidiation and during growth in medium supplemented with cell wall material from the plant pathogenic fungus Rhizoctonia solani as the sole carbon source. Expression of agl1 was supressed under iron-limiting condition, while agl1 transcript was not detected during T. atroviride interactions with the prey fungi Botrytis cinerea or R. solani. Phenotypic analysis of agl1 deletion strains (Δagl1) showed reduced conidiation compared to T. atroviride wild type, thus suggesting the involvement of AGL1 in conidiation. Furthermore, the Δagl1 strains display reduced antagonism towards B. cinerea and R. solani based on a secretion assay, although no difference was detected during direct interactions. These data demonstrate the role of AGL1 in conidiation and antagonism in the mycoparasitic fungus T. atroviride.

Aegerolysins是在原核生物和真核生物中都发现的小分泌的成孔蛋白。在真菌中，提出了aegerolysins在孢子形成，子实体形成和细胞膜裂解中的作用。本研究的目的是表征嗜酸性菌溶血素基因agl1在真菌寄生真菌木霉特罗氏木霉中的生物学功能，用于植物病害的生物防治。基因表达分析表明，在分生孢子中和生长期间，在补充有植物病原真菌茄根枯病菌作为唯一碳源的细胞壁材料的培养基中，agl1的表达较高。在铁限制条件下抑制了agl1的表达，而在T期间未检测到agl1转录本。atroviride与猎物真菌灰葡萄孢或R的相互作用。菌。与T相比，agl1缺失菌株（Δagl1）的表型分析显示降低了绝种。阿troviride野生型，因此提示AGL1参与绝种。此外，Δ AGL1菌株显示降低的拮抗作用对灰葡萄孢和- [R 。索拉尼根据分泌测定，尽管在直接相互作用期间未检测到差异。这些数据证明了AGL1在霉菌真菌T的分生和拮抗作用中的作用。阿troviride。