Environmental and intercellular Pb2+ ions determination based on encapsulated DNAzyme in nanoscale metal-organic frameworks,Microchimica Acta

当前位置： X-MOL 学术 › Microchim. Acta › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Environmental and intercellular Pb2+ ions determination based on encapsulated DNAzyme in nanoscale metal-organic frameworks
Microchimica Acta ( IF 5.3 ) Pub Date : 2020-10-14 , DOI: 10.1007/s00604-020-04586-z
Weihao Wu ₁ , Yaofang Fan ₁ , Bing Tan _{1,

2} , Huimin Zhao ₁

Affiliation

With the merits of low cost, simple synthesis procedure, and high affinity for metal ions, deoxyribozyme (DNAzyme) have played important roles in metal ions detection. However, the intracellular applications of DNAzyme are limited because of enzymatic degradation and inefficient cellular uptake. To address these problems, GR-5 as model DNAzyme was encapsulated into zeolitic imidazolate frameworks-8 (ZIF-8) nanoparticles by biomimetic mineralization. The positively charged ZIF-8 with high DNAzyme loading capacity retained their ability to enter cells. Compared with free DNAzyme, the biomimetic mineralization synthesis method has greatly improved the stability of pristine DNAzyme. The as-synthesized DNAzyme@ZIF-8 composite exhibited good stability resisting DNase I, and was used as a sensitive fluorescent nanoprobe for Pb2+ determination and successfully achieved selective and sensitive determination for Pb2+ at λex/λem = 494/522 nm in real samples. The linear range for the determination of Pb2+ is 50 to 500 nM. Moreover, the highly active DNAzyme delivered by ZIF-8 allows noninvasive imaging of Pb2+ measurement in living cells. This strategy will extend the suitability of functional nucleic acids for in vitro and in vivo bioanalysis and bioimaging. Graphical abstract Graphical abstract

中文翻译：

基于纳米金属有机框架中封装的 DNAzyme 测定环境和细胞间 Pb2+ 离子

脱氧核酶（DNAzyme）具有成本低、合成过程简单、对金属离子亲和力强等优点，在金属离子检测中发挥了重要作用。然而，由于酶促降解和细胞摄取效率低下，DNAzyme 在细胞内的应用受到限制。为了解决这些问题，通过仿生矿化将 GR-5 作为模型 DNAzyme 封装到沸石咪唑酯骨架-8 (ZIF-8) 纳米颗粒中。具有高 DNAzyme 负载能力的带正电荷的 ZIF-8 保留了它们进入细胞的能力。与游离DNAzyme相比，仿生矿化合成方法大大提高了原始DNAzyme的稳定性。合成的 DNAzyme@ZIF-8 复合物表现出良好的抗 DNase I 稳定性，并被用作用于 Pb2+ 测定的灵敏荧光纳米探针，并成功实现了对实际样品中 λex/λem = 494/522 nm 下 Pb2+ 的选择性和灵敏测定。测定 Pb2+ 的线性范围为 50 至 500 nM。此外，ZIF-8 提供的高活性 DNAzyme 允许对活细胞中的 Pb2+ 测量进行无创成像。该策略将扩展功能性核酸在体外和体内生物分析和生物成像中的适用性。图形摘要图形摘要该策略将扩展功能性核酸在体外和体内生物分析和生物成像中的适用性。图形摘要图形摘要该策略将扩展功能性核酸在体外和体内生物分析和生物成像中的适用性。图形摘要图形摘要

更新日期：2020-10-14

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11