Melipona colimana is a stingless bee endemic to the temperate regions of Jalisco, Mexico, where it provides pollinating services and is cultured for the production of honey and cerumen. There is little information about the sanitary status of M. colimana, but it is critical to know more about it to keep these bees healthy and productive. The proximity of M. colimana colonies to commercial honey bee (Apis mellifera) colonies could facilitate pathogens transmission between the two species. We determined the presence, replication, and levels of two variants of deformed wing virus (DWV-A and DWV-B), and black queen cell virus (BQCV) in samples of M. colimana and A. mellifera. We also compared the DWV-A variant with variants from other regions of the world. Both A. mellifera and M. colimana were positive to DWV-A and BQCV, but negative to DWV-B. Quantification analyses showed that A. mellifera had significantly higher levels of DWV-A and BQCV than did M. colimana. Additionally, evidence of DWV-A and BQCV replication in M. colimana was demonstrated by visualizing the minus-strand of DWV and BQCV RNA using tagged primers for RT-PCR. The DWV-A variant did not seem to be atypical compared to variants from other world regions. Due to the importance of M. colimana for the conservation of regional ecosystems as well as for its use for honey and cerumen production, the potential impact of multiplying honey bee viruses in M. colimana populations deserves further investigation.

中文翻译：

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墨西哥哈利斯科州地方性无刺蜂 Melipona colimana 变形翅病毒 A、变形翅病毒 B 和黑蜂王细胞病毒的检测、复制和定量

Melipona colimana 是墨西哥哈利斯科温带地区特有的一种无刺蜜蜂，在那里提供授粉服务，并被培养用于生产蜂蜜和耳垢。关于 M. colimana 卫生状况的信息很少，但了解更多信息对于保持这些蜜蜂的健康和生产力至关重要。M. colimana 菌落与商业蜜蜂（Apis mellifera）菌落的接近可以促进两个物种之间的病原体传播。我们确定了大肠杆菌和蜜蜂花的样本中变形翼病毒（DWV-A 和 DWV-B）和黑皇后细胞病毒 (BQCV) 的两种变体的存在、复制和水平。我们还将 DWV-A 变体与世界其他地区的变体进行了比较。A. mellifera 和 M. colimana 对 DWV-A 和 BQCV 呈阳性，但对 DWV-B 呈阴性。定量分析表明，蜜蜂花的 DWV-A 和 BQCV 水平显着高于大肠菌。此外，通过使用标记的 RT-PCR 引物对 DWV 和 BQCV RNA 的负链进行可视化，证明了 DWV-A 和 BQCV 在大肠杆菌中复制的证据。与来自世界其他地区的变体相比，DWV-A 变体似乎并不非典型。由于大肠菌对保护区域生态系统以及用于蜂蜜和耳垢生产的重要性，蜜蜂病毒在大肠菌群中繁殖的潜在影响值得进一步研究。通过使用标记的 RT-PCR 引物使 DWV 和 BQCV RNA 的负链可视化来证明大肠杆菌。与来自世界其他地区的变体相比，DWV-A 变体似乎并不是非典型的。由于大肠菌对保护区域生态系统以及用于蜂蜜和耳垢生产的重要性，蜜蜂病毒在大肠菌群中繁殖的潜在影响值得进一步研究。通过使用标记的 RT-PCR 引物使 DWV 和 BQCV RNA 的负链可视化来证明大肠杆菌。与来自世界其他地区的变体相比，DWV-A 变体似乎并不是非典型的。由于大肠菌对保护区域生态系统以及用于蜂蜜和耳垢生产的重要性，蜜蜂病毒在大肠菌群中繁殖的潜在影响值得进一步研究。