Safe disposal of petroleum oil sludge generated from crude oil storage tank bottom is a major challenge for petroleum refineries across the globe. The presence of long chain hydrocarbons in petroleum oil sludge are known to have effects on the environment through bioaccumulation or biosorption. The present study was focused to develop a modified bioremediation process using hydrocarbonoclastic microbial-assisted biocarrier matrix (MABC) mediated through biosurfactants and biocatalysts for the efficient treatment of petroleum industrial oily sludge. The development of hydrocarbonoclastic microbial-assisted biocarrier matrix was confirmed by scanning electron microscopy analysis. The biocatalysts such as lipase, laccase, esterase and biosurfactant produced by MABC system were found to be 40 U/mg, 18 U/mg, 36 U/mg and 220 mg/g of oil sludge respectively using one variable at a time approach. Further, the response surface methodology was used to determine the optimum treatment conditions (Time, pH, Mass of biocarrier matrix and Amount of oil sludge) for the enhanced removal of total petroleum hydrocarbons (TPH) present in the oil sludge and TPH was degraded by 88.78% at Hydraulic Retention Time of 7 days. The biodegradation of oil sludge was confirmed using Gas Chromatography–Mass Spectrometry analysis.

安全处置从原油储罐底部产生的石油污泥是全球石油精炼厂面临的主要挑战。已知石油污泥中长链碳氢化合物的存在会通过生物蓄积或生物吸附作用对环境产生影响。本研究的重点是开发一种改良的生物修复工艺，该工艺使用通过生物表面活性剂和生物催化剂介导的烃碎屑微生物辅助生物载体基质（MABC）来有效处理石油工业含油污泥。扫描电子显微镜分析证实了碳氢碎屑微生物辅助生物载体基质的发展。由MABC系统生产的生物催化剂，如脂肪酶，漆酶，酯酶和生物表面活性剂，分别为40 U / mg，18 U / mg，一次使用一个变量，分别产生36 U / mg和220 mg / g的油泥。此外，使用响应表面方法确定最佳处理条件（时间，pH，生物载体基质的质量和油泥的量），以提高去除油泥中总石油烃（TPH）的能力，并且TPH通过液压保持时间为7天时为88.78％。使用气相色谱-质谱分析法确认了油泥的生物降解。液压保持时间为7天时为78％。使用气相色谱-质谱分析法确认了油泥的生物降解。液压保持时间为7天时为78％。使用气相色谱-质谱分析法确认了油泥的生物降解。