Eggs oviposited by Ascaridia galli females in artificial media are commonly used as a source of infective material. We investigated the rate of egg production by cultured mature females (n = 223), and changes in egg viability under different storage and incubation conditions. Eggs recovered after 1, 2 or 3 days of culture were subjected to either (1) storage in water at 4°C (1, 4 or 8 weeks) followed by incubation in 0.1 N H2SO4 at 26°C (2, 4 or 6 weeks); or (2) prolonged storage at 4°C (up to 14 weeks). Egg development and viability was assessed by morphology coupled with a viability dye exclusion test of hatched larvae. Of the 6,044 eggs recovered per mature female 49.2, 38.5 and 12.3% were recovered on days 1, 2 and 3 of worm incubation respectively with similar initial viability (≥99%) between days. Eggs recovered on different days had only minor differences in viability after storage. The prolonged storage period at 4°C significantly affected both viability and embryonation ability resulting in decline in viability of 5.7–6.2% per week. A smaller but significant decline in egg (2.0%) and hatched larval (1.4%) viability per week of incubation at 26°C was also observed. We conclude that storage and incubation conditions, not the day of egg recovery, are the main factors affecting A. galli egg viability. Our findings indicate that under aerobic conditions storage at 26°C may be preferable to 4°C whereas other studies indicate that under anaerobic conditions storage at 4°C is preferable.

中文翻译：

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在人工培养基中回收并在不同条件下储存的鸡蛔虫卵的活力和发育

产卵的卵子鸡蛔虫人工培养基中的雌性通常被用作感染材料的来源。我们调查了培养的成熟雌性的产卵率（n= 223)，以及不同储存和孵化条件下的鸡蛋活力变化。将培养 1、2 或 3 天后回收的卵进行 (1) 在 4°C 的水中储存（1、4 或 8 周），然后在 0.1 NH 中孵育2所以4在 26°C（2、4 或 6 周）；或 (2) 在 4°C 下长时间储存​​（最多 14 周）。通过形态学结合孵化幼虫的活力染料排除试验评估卵的发育和活力。在每个成熟雌性回收的 6,044 个卵中，49.2%、38.5% 和 12.3% 分别在蠕虫孵化的第 1、2 和 3 天回收，并且在几天之间具有相似的初始存活率 (≥99%)。在不同日子回收的卵在储存后的生存力只有微小的差异。在 4°C 下延长储存期显着影响生存力和胚胎能力，导致生存力每周下降 5.7-6.2%。还观察到在 26°C 下每周孵化的卵子 (2.0%) 和孵化的幼虫 (1.4%) 存活率有较小但显着的下降。我们得出结论，储存和孵化条件，而不是鸡蛋恢复的日子，A.加利卵子活力。我们的研究结果表明，在有氧条件下储存在 26°C 可能比 4°C 更可取，而其他研究表明，在厌氧条件下储存在 4°C 更可取。