Microbial adhesion is critical for parasitic infection and colonization of host cells. To study the host–parasite interaction in vitro, we established a flow cytometry-based assay to measure the adherence of Trichomonas vaginalis to epithelial cell line SiHa. SiHa cells and T. vaginalis were detected as clearly separated, quantifiable populations by flow cytometry. We found that T. vaginalis attached to SiHa cells as early as 30 min after infection and the binding remained stable up to several hours, allowing for analysis of drug treatment efficacy. Importantly, NADPH oxidase inhibitor DPI treatment induced the detachment of T. vaginalis from SiHa cells in a dose-dependent manner without affecting host cell viability. Thus, this study may provide an understanding for the potential development of therapies against T. vaginalis and other parasite infections.

中文翻译：

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联苯胺碘鎓抑制阴道毛滴虫对SiHa细胞的粘附

微生物粘附对于寄生虫感染和宿主细胞定殖至关重要。为了研究体外宿主与寄生虫之间的相互作用，我们建立了基于流式细胞仪的检测方法，以测量阴道滴虫对上皮细胞系SiHa的粘附。通过流式细胞术将SiHa细胞和阴道锥虫检测为明显分离的，可量化的种群。我们发现，在感染后30分钟之内，阴道隐孢子虫就附着在SiHa细胞上，其结合在数小时内保持稳定，从而可以分析药物治疗的功效。重要的是，NADPH氧化酶抑制剂DPI处理诱导了阴道锥虫的脱离从SiHa细胞中以剂量依赖的方式产生，而不影响宿主细胞的活力。因此，该研究可以为针对阴道毛滴虫和其他寄生虫感染的疗法的潜在发展提供理解。