The Panamanian rocket frog Colostethus panamansis (family Dendrobatidae) has been affected by chytridiomycosis, a deadly disease caused by the fungus Batrachochytrium dendrobatidis (Bd). While there are still uninfected frogs, we set out to isolate microbes from anatomically distinct regions in an effort to create a cultivable resource within Panama for potential drug/agricultural/ecological applications that perhaps could also be used as part of a strategy to protect frogs from infections. To understand if there are specific anatomies that should be explored in future applications of this resource, we mapped skin-associated bacteria of C. panamansis and their metabolite production potential by mass spectrometry on a 3D model. Our results indicate that five bacterial families (Enterobacteriaceae, Comamonadaceae, Aeromonadaceae, Staphylococcaceae and Pseudomonadaceae) dominate the cultivable microbes from the skin of C. panamansis. The combination of microbial classification and molecular analysis in relation to the anti-Bd inhibitory databases reveals the resource has future potential for amphibian conservation.

中文翻译：

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从巴拿马火箭蛙 Colostethus panamansis（无尾： Dendrobatidae）皮肤中分离出的微生物的代谢物

巴拿马箭蛙Colostethus panamansis（Dendrobatidae 科）已受到壶菌病的影响，这是一种由真菌Batrachochytrium dendrobatidis ( Bd ) 引起的致命疾病。虽然仍然有未受感染的青蛙，但我们着手从解剖学上不同的区域分离微生物，努力在巴拿马境内创造可耕种资源，用于潜在的药物/农业/生态应用，这也许也可以用作保护青蛙免受感染的战略的一部分感染。为了了解在该资源的未来应用中是否应该探索特定的解剖结构，我们通过质谱法在 3D 模型上绘制了巴拿马 C. panamansis的皮肤相关细菌及其代谢物生产潜力。我们的结果表明，五个细菌科（肠杆菌科、丛毛单胞菌科、气单胞菌科、葡萄球菌科和假单胞菌科）在巴拿马 C. panamansis皮肤的可培养微生物中占主导地位。微生物分类和与抗Bd抑制数据库相关的分子分析相结合，揭示了该资源未来具有两栖动物保护潜力。