Abstract

Objective The alteration in certain trace elements is usually associated with impaired immune function and higher oxidative stress. Therefore, these elements are suggested to play an important role in the pathogenesis of neonatal sepsis. We aimed to evaluate copper (Cu), zinc (Zn), and selenium (Se) serum levels in full-term neonates with late-onset sepsis (LOS) and correlate these levels with DNA damage and other risk factors of sepsis.

Methods The study included a group of 100 neonates diagnosed with sepsis serving as the case group and another one of 60 neonates serving as the control group. DNA damage was assessed using the comet assay method and trace elements were measured using inductively coupled plasma mass spectrometry.

Results Compared with controls, the percentage of DNA damage was significantly elevated in patients with sepsis, while serum levels of Cu, Zn, and Se were markedly decreased (p = 0.001). A strong negative correlation was revealed between Se and DNA damage (r = −0.6, p = 0.001). However, no correlations were found between Cu or Zn and DNA damage. Univariate logistic regression analysis revealed that DNA damage as well as Cu, Zn, and Se serum levels can be considered as relevant risk factors for neonatal sepsis (p = 0.008, 0.004, 0.004, and 0.003, respectively). Receiver-operating characteristic curve analysis showed that the strongest indicator for neonatal sepsis was Se (area under the curve [AUC] = 0.94, confidence interval [CI] = 0.9–0.98, p = 0.001), followed by Cu (AUC = 0.9, CI = 0.85–0.96, p = 0.001), and then Zn (AUC = 0.87, CI = 0.8–0.93, p = 0.001).

Conclusion The percentage of DNA damage may help in the assessment of neonatal sepsis severity. Altered levels of Cu, Zn, and Se may play significant role in the pathogenesis of neonatal sepsis. Se serum level is strongly correlated with percentage of DNA damage. Therefore, Se can predict the severity of LOS.

Publication History

Received: 11 April 2020

Accepted: 18 August 2020

Publication Date:
12 October 2020 (online)

© 2020. Thieme. All rights reserved.

Georg Thieme Verlag KG
Stuttgart · New York

中文翻译：

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晚期脓毒症足月新生儿DNA损伤与血清铜，锌和硒水平的关联

摘要

目的 某些微量元素的改变通常与免疫功能受损和较高的氧化应激有关。因此，建议这些元素在新生儿败血症的发病机理中起重要作用。我们旨在评估患有迟发性败血症（LOS）的足月新生儿的铜（Cu），锌（Zn）和硒（Se）血清水平，并将这些水平与DNA损伤和败血症的其他危险因素相关联。

方法 该研究包括100例诊断为败血症的新生儿为病例组，另60例新生儿为对照组。使用彗星分析方法评估DNA损伤，并使用电感耦合等离子体质谱法测量痕量元素。

结果 与脓毒症患者相比，DNA损伤百分比显着升高，而血清中的Cu，Zn和Se含量则明显降低（p  = 0.001）。硒和DNA损伤之间存在强烈的负相关性（r  = -0.6，p  = 0.001）。但是，在铜或锌与DNA损伤之间未发现相关性。单因素Logistic回归分析显示DNA损伤以及Cu，Zn和Se血清水平可被视为新生儿败血症的相关危险因素（p 分别为0.008、0.004、0.004和0.003）。接收者操作特征曲线分析显示，新生儿败血症的最强指标是Se（曲线下面积[AUC] = 0.94，置信区间[CI] = 0.9–0.98，p  = 0.001），其次是Cu（AUC = 0.9， CI = 0.85-0.96，p  = 0.001），然后是Zn（AUC = 0.87，CI = 0.8-0.93，p  = 0.001）。

结论 DNA损伤的百分比可能有助于评估新生儿败血症的严重程度。铜，锌和硒含量的变化可能在新生儿败血症的发病机理中起重要作用。血清硒水平与DNA损伤百分比密切相关。因此，Se可以预测LOS的严重程度。

出版历史

收到：2020年4月11日

接受：2020年8月18日

发布日期：
2020年10月12日（在线）

©2020年。Thieme。版权所有。

Georg Thieme Verlag KG
斯图加特·纽约