The current study aims to evaluate the modulatory effect of zinc oxide nanoparticles (ZnO NPs) on the bioenergetic signature biomarkers in the Ehrlich ascitic carcinoma (EAC) model. To achieve this goal, 90 female albino mice were included in this study and were divided into six equal groups (n =15 per group): saline‐treated group, ZnO NP‐treated, EACs‐bearing mice, and three groups of EACs‐bearing mice treated with ZnO NPs at a dose of 20 mg/kg every other day, 10 mg/kg every other day, 10 mg/kg every day, respectively, for 14 days. The tissues from treated groups and control groups were homogenized and used for the assay of glyceraldehyde 3‐phosphate dehydrogenase (GAPDH) and F1 beta subunit of adenosine triphosphate (ATP) synthase levels, as well as the determination of lactate level. The survival time of mice was improved in all ZnO NP‐treated groups, especially in EACs‐bearing mice treated with ZnO NPs at a dose of 10 mg/kg every other day. This improvement was associated with an increased F1 beta subunit of ATP synthase level and a decreased GAPDH level. Also, the lactate level was significantly decreased in all treated groups when compared with the untreated group. The overall effect was the increased bioenergetic signature as compared with EC.These results implied that ZnO NPs have a significant efficacy against cancer cells and they significantly increased the bioenergetic signature.

中文翻译：

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生物能标记作为埃希氏腹水癌小鼠中氧化锌纳米粒子的靶标

当前的研究旨在评估氧化锌纳米颗粒（ZnO NPs）对Ehrlich腹水癌（EAC）模型中生物能标记生物标志物的调节作用。为了实现这一目标，本研究包括了90只雌性白化病小鼠，并分为六个相等的组（每组n = 15）：盐水处理组，ZnO NP处理，带有EAC的小鼠以及三组EAC-每隔一天分别以20 mg / kg，每隔一天10 mg / kg，每天10 mg / kg的剂量分别用ZnO NPs治疗的小鼠持续14天。将来自治疗组和对照组的组织均质化，并用于测定甘油三磷酸腺苷脱氢酶（GAPDH）和三磷酸腺苷（ATP）合酶的F1β亚基，以及测定乳酸水平。在所有经ZnO NP治疗的组中，小鼠的存活时间均得到改善，尤其是隔日以10 mg / kg剂量的ZnO NP治疗的带有EAC的小鼠。这种改善与ATP合酶水平的F1β亚基升高和GAPDH水平降低有关。此外，与未治疗组相比，所有治疗组的乳酸水平均显着降低。总体效果是与EC相比增加了生物能签名。这些结果表明ZnO NP对癌细胞具有显着的功效，并且可以显着提高生物能签名。与未治疗组相比，所有治疗组的乳酸水平均显着降低。总体效果是与EC相比增加了生物能签名。这些结果表明ZnO NP对癌细胞具有显着的功效，并且可以显着提高生物能签名。与未治疗组相比，所有治疗组的乳酸水平均显着降低。总体效果是与EC相比增加了生物能签名。这些结果表明ZnO NP对癌细胞具有显着的功效，并且可以显着提高生物能签名。