Selectin ligands are present on the surface of tumor cells, for this reason lowering the L‐selectin level in the blood and lymph can indicate presence of the tumor. Therefore the selectin level in the plasma are potential targets for anticancer therapy. We demonstrate the surface enhanced Raman spectroscopy (SERS)‐based sensor for the determination of L‐selectin level in biological samples that can be used in medical diagnosis. The combination of SERS with the method of multivariate analysis as principle component analysis (PCA) allows to strengthen the presented data analysis. The loadings of PCA permit to indicate those vibration modes, that are the most important for the assumed identification (bands at 1574, 1450, 1292 cm⁻¹). Two bands at 1286 and 1580 cm⁻¹ were selected for the determination of the calibration curve (bands intensities I₁₂₈₆/I₁₅₈₀ ratio). The L‐selectin level of biological samples can be read, directly from the calibration curve. The presented sensor is as a sensitive tool with good specificity and selectivity of L‐selectin, even in the case of coexistence of P‐ and E‐selectin.

中文翻译：

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用于直接测定血浆样品中 L-选择素水平的基于 SERS 的传感器作为肿瘤检测的替代方法

选择素配体存在于肿瘤细胞表面，因此降低血液和淋巴液中的 L-选择素水平可以表明肿瘤的存在。因此，血浆中的选择素水平是抗癌治疗的潜在目标。我们展示了基于表面增强拉曼光谱 (SERS) 的传感器，用于测定生物样品中的 L-选择素水平，可用于医学诊断。SERS 与作为主成分分析 (PCA) 的多变量分析方法的结合可以加强所呈现的数据分析。PCA 的载荷允许指示那些对于假设识别最重要的振动模式（1574、1450、1292 cm ^-1的波段）。1286 和 1580 cm ^{-1的两个波段}选择用于确定校准曲线（条带强度 I ₁₂₈₆ /I ₁₅₈₀比率）。可以直接从校准曲线中读取生物样品的 L-选择素水平。所提出的传感器是一种灵敏的工具，对 L-选择素具有良好的特异性和选择性，即使在 P-和 E-选择素共存的情况下也是如此。