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NITROGEN RESPONSE DEFICIENCY 1‐mediated CHL1 induction contributes to optimized growth performance during altered nitrate availability in Arabidopsis
The Plant Journal ( IF 6.2 ) Pub Date : 2020-10-13 , DOI: 10.1111/tpj.15007
Won J Lee 1, 2 , Hai A Truong 1 , Cao S Trịnh 1 , Jun H Kim 1 , Seokjin Lee 1 , Suk-Whan Hong 3 , Hojoung Lee 1, 2
Affiliation  

Plants cannot fix nitrogen directly; they must absorb it from the soil through their roots, or in rare cases, form associations with nitrogen‐fixing bacteria. The efficiency of nitrogen use in most domesticated crops is low, and more than half of the available nitrogen in the soil can leach into the environment. Understanding the nitrogen signaling pathways is essential for maximizing the efficiency of nitrogen use in crops. In the present study, we characterized the Myeloblastosis (Myb)‐like gene NITROGEN RESPONSE DEFICIENCY 1 (NID1). We observed that the growth performance of nid1 knockout (KO) mutant Arabidopsis plants was better than that of wild‐type Col‐0 plants under very low‐nitrate conditions, leading to improved growth performance in the nid1 KO plants. The results of chromatin immunoprecipitation and electrophoretic mobility shift assays indicated that NID1 binds to the promoter of the NITRATE TRANSPORTER (NRT)1.1 gene. Furthermore, nid1 KO plants exhibited similar growth performance to the nid1 KO/chl1‐5 (nrt1.1 KO) double mutant and chl1‐5 (nrt1.1 KO) plants in response to low‐nitrate conditions. We suggest that NID1 plays a crucial role as a transcription factor in optimizing plant growth by modulating the transcript abundance of the nitrate transceptor CHL1, leading to enhanced ABA accumulation in low‐nitrate conditions.

中文翻译:

氮响应缺陷1介导的CHL1诱导在拟南芥中改变硝酸盐有效性的过程中有助于优化生长性能

植物不能直接固氮。他们必须通过其根部从土壤吸收它,或者在极少数情况下,它们会与固氮细菌结合。大多数驯化作物中的氮利用效率很低,土壤中超过一半的可用氮会渗入环境。了解氮的信号传导途径对于最大化作物中氮的利用效率至关重要。在本研究中,我们表征了成髓细胞瘤的Myb) -样基因氮RESPONSE亏1NID1)。我们观察到nid1的生长性能基因敲除(KO)突变拟南芥植物在非常低的硝酸盐条件下要优于野生型Col-0植物,从而改善了nid1 KO植物的生长性能。染色质免疫沉淀和电泳迁移率变动分析的结果表明,NID1与NITRATE TRANSPORTERNRT)1.1基因的启动子结合。此外,nid1 KO植物表现出与nid1 KO / chl1-5nrt1.1 KO)双突变体和chl1-5nrt1.1)类似的生长性能。KO)对低硝酸盐条件的响应。我们建议NID1扮演在通过调节硝酸盐transceptor的转录本丰度优化植物生长的转录因子至关重要的作用CHL1,导致低硝酸盐条件增强ABA的积累。
更新日期:2020-12-01
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