Gene editing and/or allele introgression with absolute precision and control appear to be the ultimate goals of genetic engineering. Precision genome editing in plants has been developed through various approaches, including oligonucleotide‐directed mutagenesis (ODM), base editing, prime editing and especially homologous recombination (HR)‐based gene targeting. With the advent of CRISPR/Cas for the targeted generation of DNA breaks (single‐stranded breaks (SSBs) or double‐stranded breaks (DSBs)), a substantial advancement in HR‐mediated precise editing frequencies has been achieved. Nonetheless, further research needs to be performed for commercially viable applications of precise genome editing; hence, an alternative innovative method for genome editing may be required. Within this scope, we summarize recent progress regarding precision genome editing mediated by microhomology‐mediated end joining (MMEJ) and discuss their potential applications in crop improvement.

中文翻译：

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通过微同源性介导的末端连接进行基于CRISPR / Cas的精确基因组编辑

具有绝对精度和控制力的基因编辑和/或等位基因渗入似乎是基因工程的最终目标。植物的精确基因组编辑已通过多种方法开发，包括寡核苷酸定向诱变（ODM），碱基编辑，主要编辑，尤其是基于同源重组（HR）的基因定位。随着CRISPR / Cas的出现，靶向产生DNA断裂（单链断裂（SSB）或双链断裂（DSB）），在HR介导的精确编辑频率方面取得了实质性进展。但是，对于精确基因组编辑在商业上可行的应用，还需要进行进一步的研究。因此，可能需要另一种创新的基因组编辑方法。在这个范围内