Histone mRNA is subject to 3′ uridylation and re‐adenylation in Aspergillus nidulans,Molecular Microbiology

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Histone mRNA is subject to 3′ uridylation and re‐adenylation in Aspergillus nidulans
Molecular Microbiology ( IF 2.6 ) Pub Date : 2020-10-12 , DOI: 10.1111/mmi.14613
Amir Mossanen-Parsi ₁ , Daniele Parisi _{1,

2} , Natasha Browne-Marke ₃ , Izwan Bharudin ₁ , Sean R Connell _{2,

4} , Olga Mayans ₁ , Paola Fucini _{2,

4} , Igor Y Morozov _{1,

3} , Mark X Caddick ₁

Affiliation

The role of post‐transcriptional RNA modification is of growing interest. One example is the addition of non‐templated uridine residues to the 3′ end of transcripts. In mammalian systems, uridylation is integral to cell cycle control of histone mRNA levels. This regulatory mechanism is dependent on the nonsense‐mediated decay (NMD) component, Upf1, which promotes histone mRNA uridylation and degradation in response to the arrest of DNA synthesis. We have identified a similar system in Aspergillus nidulans, where Upf1 is required for the regulation of histone mRNA levels. However, other NMD components are also implicated, distinguishing it from the mammalian system. As in human cells, 3′ uridylation of histone mRNA is induced upon replication arrest. Disruption of this 3′ tagging has a significant but limited effect on histone transcript regulation, consistent with multiple mechanisms acting to regulate mRNA levels. Interestingly, 3′ end degraded transcripts are also subject to re‐adenylation. Both mRNA pyrimidine tagging and re‐adenylation are dependent on the same terminal‐nucleotidyltransferases, CutA, and CutB, and we show this is consistent with the in vitro activities of both enzymes. Based on these data we argue that mRNA 3′ tagging has diverse and distinct roles associated with transcript degradation, functionality and regulation.

中文翻译：

组蛋白 mRNA 在构巢曲霉中发生 3' 尿苷化和再腺苷酸化

转录后 RNA 修饰的作用越来越受到关注。一个例子是在转录本的 3' 端添加非模板化的尿苷残基。在哺乳动物系统中，尿苷化是组蛋白 mRNA 水平的细胞周期控制不可或缺的一部分。这种调节机制依赖于无义介导的衰变 (NMD) 成分 Upf1，它促进组蛋白 mRNA 尿苷化和降解，以响应 DNA 合成的停滞。我们在构巢曲霉中发现了一个类似的系统，其中 Upf1 是调节组蛋白 mRNA 水平所必需的。然而，其他 NMD 组件也有牵连，将其与哺乳动物系统区分开来。与人类细胞一样，组蛋白 mRNA 的 3' 尿苷化在复制停滞时被诱导。这种 3' 标记的破坏对组蛋白转录调控具有显着但有限的影响，这与调节 mRNA 水平的多种机制一致。有趣的是，3'端降解的转录本也受到再腺苷酸化的影响。mRNA 嘧啶标记和再腺苷酸化都依赖于相同的末端核苷酸转移酶 CutA 和 CutB，我们表明这与两种酶的体外活性一致。基于这些数据，我们认为 mRNA 3' 标记具有与转录本降解、功能和调节相关的多种不同的作用。

更新日期：2020-10-12

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