The behaviour of mitoxantrone (MTX), an anthracenedione antineoplastic agent, in different types of organized medium was explored using molecular spectrofluorometry. The original fluorescence and quantum yield of MTX were augmented by about five‐fold in the aqueous buffered solution (Britton–Robinson, pH 3.0) by the addition of sodium dodecyl sulfate. Enhancement in the fluorescence intensity did not come from the boost in the ultraviolet (UV) light absorbance of the drug in the presence of micelles but due to shielding of the lowest excited singlet state of the drug from a radiationless process inside the cavity of the micelle. Accordingly, a versatile, sensitive, and feasible spectrofluorimetric method was constructed and evaluated for MTX determination. Fluorescence measurements were performed at 675 nm (λ_ex 610 nm). A linear relationship was shown between fluorescence intensity and drug concentration within the range 0.01–2.0 μg ml⁻¹ of MTX with a correlation coefficient of 0.9999 and a detection limit of 2 ng ml⁻¹. The developed method was effectively used for analysis of MTX in biological samples and dosage forms. In addition, the method was expanded to study the stability of MTX exposed to different drastic degradations and the kinetic parameters of the degradation were calculated.

中文翻译：

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分光光度法研究米托蒽醌与阴离子表面活性剂的相互作用及其在药物制剂和生物液体的可行性分析中的应用

使用分子荧光光谱法研究了蒽酮二酮类抗肿瘤药米托蒽醌（MTX）在不同类型的有组织培养基中的行为。通过添加十二烷基硫酸钠，在缓冲水溶液（Britton-Robinson，pH 3.0）中，MTX的原始荧光和量子产率提高了大约五倍。荧光强度的增强不是由于存在胶束时药物对紫外线（UV）吸光度的提高，而是由于屏蔽了胶束腔内的无辐射过程，从而使药物的最低激发单重态受到屏蔽。因此，构建了一种通用，灵敏且可行的荧光分光光度法，并对其进行了MTX测定进行了评估。荧光测量在675 nm（_λex610 nm）。荧光强度与药物浓度在MTX 0.01–2.0μgml ^-1范围内呈线性关系，相关系数为0.9999，检出限为2 ng ml ^-1。所开发的方法有效地用于分析生物样品和剂型中的MTX。另外，扩展了该方法以研究MTX在不同剧烈降解下的稳定性，并计算了降解的动力学参数。