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Activity‐Based Protein Profiling of Chitin Catabolism
ChemBioChem ( IF 3.2 ) Pub Date : 2020-10-13 , DOI: 10.1002/cbic.202000616
Elias K Zegeye 1, 2 , Natalie C Sadler 2 , Gerard X Lomas 2 , Isaac K Attah 3 , Janet K Jansson 2 , Kirsten S Hofmockel 2, 4 , Christopher R Anderton 3 , Aaron T Wright 1, 2
Affiliation  

Trying to fit in chitin: To understand microbial chitinase function, we synthesized activity‐based probes by modifying the chitin derivatives chitotriose and N‐acetylglucosamine. The probes were used to profile the functional response of Cellvibrio japonicus to culture on chitin and non‐chitin substrates. Fluorescence gels and proteomics were used to characterize probe labeling.
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中文翻译:

几丁质分解代谢的基于活性的蛋白质分析

尝试适应几丁质:为了了解微生物几丁质酶的功能,我们通过修饰几丁质衍生物壳三糖和N-乙酰氨基葡萄糖合成了基于活性的探针。这些探针用于分析Cellvibrio japonicus对几丁质和非几丁质底物培养的功能反应。荧光凝胶和蛋白质组学用于表征探针标记。
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更新日期:2020-10-13
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