Circadian rhythms are generated by a circadian clock for which oscillations are based on the rhythmic expression of the so-called clock genes. The present study investigated the role of Gryllus bimaculatus vrille (Gb’vri) and Par domain protein 1 (Gb’Pdp1) in the circadian clock of the cricket Gryllus bimaculatus. Structural analysis of Gb’vri and Gb’Pdp1 cDNAs revealed that they are a member of the bZIP transcription factors. Under light/dark cycles (LD) both genes were rhythmically expressed in the clock tissue, the optic lobes, whereas the rhythm diminished under constant darkness (DD). Gb’vri and Gb’Pdp1 mRNA levels were significantly reduced by RNA interference (RNAi) of Gb’Clk and Gb’cyc, suggesting they are controlled by Gb’CLK/Gb’CYC. RNAi of Gb’vri and Gb’Pdp1 had little effect on locomotor rhythms, although their effects became visible when treated together with Gb’cyc^RNAi. The average free-running period of Gb’vri^RNAi/Gb’cyc^RNAi crickets was significantly shorter than that of Gb’cyc^RNAi crickets. A similar period shortening was observed also when treated with Gb’Pdp1^RNAi/Gb’cyc^RNAi. Some Gb’Pdp1^RNAi/Gb’cyc^RNAi crickets showed rhythm splitting into two free-running components with different periods. Gb’vri^RNAi and Gb’Pdp1^RNAi treatments significantly altered the expression of Gb’Clk, Gb’cyc, and Gb’tim in LD. These results suggest that Gb’vri and Gb’Pdp1 play important roles in cricket circadian clocks.

昼夜节律由昼夜节律时钟产生，其振荡基于所谓的时钟基因的节律表达。本研究调查了ry Gryllus bimaculatus vrille（Gb'vri）和Par结构域蛋白1（Gb'Pdp1）在the Gryllus bimaculatus昼夜节律中的作用。结构分析千兆“ VRI和千兆” PDP1的cDNA透露，他们是bZIP类转录因子的成员。在明/暗周期（LD）下，两个基因均在钟表组织（视叶）中有节奏地表达，而在恒定黑暗（DD）下节奏则减弱。千兆位“ VRI和Gb'Pdp1 mRNA水平的RNA干扰（RNAi）的显著降低千兆” Clk时和千兆“ CYC，这表明它们是由控制千兆” CLK /千兆” CYC。RNA干扰Gb'vri和Gb'Pdp1对运动的节奏影响不大，但是当一起处理它们的效果开始显现千兆' CYC ^RNA干扰。的平均自由运行周期Gb'vri^的RNAi /千兆' CYC^的RNAi蟋蟀比的显著短Gb'cyc ^RNAi cket。也观察到了类似的周期缩短时与处理Gb'Pdp1^的RNAi /千兆' CYC^的RNAi。有些Gb'Pdp1^的RNAi /千兆' CYC ^RNAi的蟋蟀表明节奏分裂成不同周期两个自由运行的组件。Gb'vri^的RNAi和Gb'Pdp1^的RNAi治疗显著改变的表达千兆“ Clk时，千兆” CYC，和千兆'添在LD。这些结果表明Gb'vri和Gb'Pdp1在板球生物钟中扮演重要角色。