Efficient and Clean Preparation of Rare Prosaikogenin D by Enzymatic Hydrolysis of Saikosaponin B2 and Response Surface Methodology Optimization,Enzyme and Microbial Technology

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Efficient and Clean Preparation of Rare Prosaikogenin D by Enzymatic Hydrolysis of Saikosaponin B2 and Response Surface Methodology Optimization
Enzyme and Microbial Technology ( IF 3.4 ) Pub Date : 2020-12-01 , DOI: 10.1016/j.enzmictec.2020.109690
Mengru Wu ₁ , Man Wang ₁ , Yangyang Zhang ₁ , Jinwei Zhou ₁ , Lili Xu ₁ , Guohua Xia ₁ , Zhipeng Yan ₂ , Yuping Shen ₁ , Huan Yang ₁

Affiliation

Prosaikogenin D, a rare secondary saponin in Radix Bupleuri, has much higher in vivo bioactivities than its original glycoside saikosaponin B2. Its preparation methods, such as conventional acid hydrolysis and column chromatograph, are unfriendly to environment with serious pollution and undesired products. The aim of this study was to establish an efficient and clean approach for convenient preparation of this rare steroid saponin based on the enzymatic hydrolysis. Cellulase was selected from four commercial enzymes due to its higher hydrolysis performance. Then the hydrolysis conditions were optimized by response surface methodology after preliminary investigation on affecting factors by single-factor experiments. The reaction system was constructed by 100 μg/mL of saikosaponin B2 and 8.00 mg/mL of cellulase, which was incubated in HAc-NaAc buffer (pH 4.7) at 60 °C for 33 h. Consequently, a high conversion ratio of the substrate has been achieved at 95.04 %. The newly developed strategy is an efficient and clean approach for the preparation of prosaikogenin D and it is a promising technology in industrial application.

中文翻译：

柴胡皂苷B2酶解高效清洁制备稀有原柴胡皂苷元D及响应面方法优化

Prosaikogenin D 是柴胡中罕见的次生皂苷，其体内生物活性远高于其原始苷柴胡皂苷 B2。其常规酸水解、柱色谱等制备方法对环境不友好，污染严重，生成副产品。本研究的目的是建立一种高效、清洁的方法，基于酶水解方便地制备这种稀有甾体皂苷。由于其较高的水解性能，纤维素酶选自四种商业酶。通过单因素试验初步考察影响因素后，采用响应面法优化水解条件。反应体系由100 μg/mL柴胡皂苷B2和8.00 mg/mL纤维素酶构建，将其在 HAc-NaAc 缓冲液 (pH 4.7) 中于 60°C 孵育 33 小时。因此，基板的高转化率达到了 95.04%。新开发的策略是制备 prosaikogenin D 的一种高效、清洁的方法，是一种很有前途的工业应用技术。

更新日期：2020-12-01

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