A feeding trial was conducted to investigate the effects of Chinese herbal medicines mixture (CHMM) on growth performance, digestive enzyme activity and serum biochemical parameters of European eels, (initial weight 6.19±0.09 g). Diets with different supplementation of CHMM (0, 2.5, 5, 7.5, 10, 12.5 g/kg) were given to eels for 42 days. Results showed: eels fed with 5–12.5 g/kg CHMM were observed an improvement in growth performance, where the weight gain (WG) and specific growth rate (SGR) were significantly enhanced. Eels fed with 5–12.5 g/kg CHMM presented a higher activity of enteric trypsin (TRS) (P<0.05). Meanwhile, an enhancement of amylase activity was observed in eels fed with 10 g/kg CHMM (P<0.05). Eels fed with 10 g/kg CHMM was observed a marked improvement in body protein content. The total superoxide dismutase activity (T-SOD) had a significant improvement among all experimental groups compared with the control group, and the lysozyme activity (LZM) was also enhanced by dietary 5 and 7.5 g/kg CHMM addition (P<0.05). For determine the optimal addition of CHMM, WG, SGR, TRS, body protein content, T-SOD and LZM were choose to carry out regression analysis. Regression analysis results show, 7.422, 7.343, 9.156, 9.085, 4.465 and 7.282 g/kg CHMM are the optimal addition for those parameters, respectively. In conclusion, dietary CHMM could improve the growth performance, enteric trypsin activity, body protein content, non-specific immunity and antioxidant capacity of eels, and the recommended supplementation of CHMM for European eels is about 9 g/kg.

中文翻译：

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日粮中药合剂对欧洲鳗、鳗鲡生长性能、消化酶活性及血清生化参数的影响


通过饲养试验，研究中草药合剂（CHMM）对欧洲鳗鱼（初始体重6.19±0.09 g）生长性能、消化酶活性和血清生化参数的影响。鳗鱼饲粮中添加不同CHMM（0、2.5、5、7.5、10、12.5 g/kg），连续42天。结果显示：饲喂 5-12.5 g/kg CHMM 的鳗鱼生长性能有所改善，增重 (WG) 和特定生长率 (SGR) 显着提高。饲喂5-12.5 g/kg CHMM的鳗鱼肠胰蛋白酶（TRS）活性较高（P<0.05）。同时，饲喂10 g/kg CHMM的鳗鱼淀粉酶活性增强（P<0.05）。观察到喂食 10 g/kg CHMM 的鳗鱼体内蛋白质含量显着提高。与对照组相比，各实验组总超氧化物歧化酶活性（T-SOD）均有显着提高，且日粮中添加5和7.5 g/kg CHMM也提高了溶菌酶活性（LZM）（P<0.05）。为了确定CHMM的最佳添加量，选择WG、SGR、TRS、机体蛋白含量、T-SOD和LZM进行回归分析。回归分析结果显示，7.422、7.343、9.156、9.085、4.465和7.282 g/kg CHMM分别是这些参数的最佳添加量。综上所述，日粮中添加CHMM可提高鳗鱼的生长性能、肠胰蛋白酶活性、体内蛋白质含量、非特异性免疫和抗氧化能力，欧洲鳗鱼推荐添加CHMM约为9 g/kg。