Mongolian sheep and Dorper sheep as keystone sheep breed present excellent adaptability to the surroundings, but the heat-responsive genes of two sheep breeds are rarely explored, especially non-coding RNA gene. The three Mongolian sheep and three Dorper sheep were treated under heat condition, and the livers of six sheep were sampled and arranged to sequence. Using bioinformatics analysis to investigate RNA-seq data, 335 differentially expressed protein-coding genes and 65 long non-coding RNA (lncRNA) genes were emerged. In the liver of Mongolian sheep, 5 protein-coding genes relevant to heat response were inspected, including COMMD10, ABCA9, NIPAL1, APOA4 and LIN7A while 8 genes containing NUP62, PLIN2, ACACB, NIPAL1, EXO1, LGALS3, TRPM1 and TRPV2 were screened in liver of Dorper sheep. The biological effects of the 12 protein-coding genes were involved in heat shock protein binding, lipid homeostasis and storage, fatty acid oxidation, magnesium/calcium ions import, anti-oxidation and DNA repair. Among above heat response-centric genes, ABCA9 was predicted as target gene of significantly differential XLOC_476131. The selected 12 protein-coding genes and 1 lncRNA genes were considered to provide an understanding of how sheep positively respond to heat stress, and further to provide molecular evidence on heat-tolerance.

蒙古绵羊和杜泊绵羊作为基石绵羊品种对周围环境具有很好的适应性，但是很少探索两个绵羊品种的热响应基因，特别是非编码RNA基因。在加热条件下处理了三只蒙古绵羊和三只杜泊绵羊，并对六只绵羊的肝脏取样并安排了测序。使用生物信息学分析研究RNA序列数据，出现了335个差异表达的蛋白质编码基因和65个长的非编码RNA（lncRNA）基因。在蒙古绵羊的肝脏中，检查了5个与热反应相关的蛋白质编码基因，包括COMMD10，ABCA9，NIPAL1，APOA4和LIN7A，而8个基因包含NUP62，PLIN2，ACACB，NIPAL1，EXO1，LGALS3，TRPM1和在杜泊绵羊肝脏中筛选了TRPV2。12种蛋白质编码基因的生物学效应涉及热激蛋白结合，脂质稳态和储存，脂肪酸氧化，镁/钙离子输入，抗氧化和DNA修复。在上述以热反应为中心的基因中，ABCA9被预测为XLOC_476131显着差异的靶基因。选择的12个蛋白质编码基因和1个lncRNA基因被认为有助于了解绵羊如何对热应激作出积极反应，并进一步提供耐热性的分子证据。