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A new morphological analysis of eggs and miracidia of Paratanaisia bragai (Digenea: Eucotylidae)
Biologia ( IF 1.4 ) Pub Date : 2020-10-13 , DOI: 10.2478/s11756-020-00604-w
Vanessa Barreto Xavier , Aleksandra Oliveira-Menezes , Viviane Sant’anna de Souza , Solange Viana Paschoal Blanco Brandolini , Wanderley de Souza , Renato A. DaMatta , Jairo Pinheiro

Paratanaisia bragai is a trematode that reaches sexual maturity in the kidney collecting ducts of domestic and wild birds, while the gastropods Subulina octona and Leptinaria unilamellata serve as its intermediate hosts. The morphology of P. bragai eggs is described here for the first time, using light, scanning and transmission electron microscopy. Embryonic eggs, obtained from adult worms collected from naturally infected definitive hosts, appeared elliptical, with an evident operculum, by all three microscopic techniques employed. Scanning electron microscopy revealed that the abopercular region presents a prominent U-shaped knob, which stands out from the egg surface. The thickness of the eggs, measured from ultrathin sections, were 0.34 ± 0.06 µm (0.29–0.50) at the ends and 0.31 ± 0.05 µm (0.24–0.36) in the middle region. The internal shell layer was more electron-dense and thinner than the middle layer, which comprised nearly the entire shell, while the external layer was less electron-dense and thinner than the internal layer. Rupture of the eggs, by the coverslip pressure, revealed the miracidia of P. bragai that was characterized by light and transmission electron microscopy.



中文翻译:

一种新的鸡蛋和鹦鹉螺的嗜酸性的形态学分析(双基因:Eucotylidae)

Bratanai是一种吸虫,在家禽和野禽的肾脏收集管中达到性成熟,而腹足纲的Subulina octonaLeptinaria unilamellata充当其中间宿主。百日咳杆菌的形态首次使用光,扫描和透射电子显微镜对卵进行了描述。通过使用的所有三种显微镜技术,从从自然感染的定性宿主收集的成年蠕虫获得的胚卵呈椭圆形,并带有明显的cul盖。扫描电镜观察发现,在癌旁区域有一个突出的U形旋钮,从蛋表面突出。从超薄切片测得的鸡蛋厚度在末端为0.34±0.06 µm(0.29–0.50),在中间区域为0.31±0.05 µm(0.24–0.36)。内壳层的电子密度更高,并且比几乎包括整个壳层的中间层更薄,而外层的电子密度更低,也比内层薄。卵破裂,通过盖玻片压力,通过光和透射电子显微镜表征的布拉加毕赤酵母

更新日期:2020-10-13
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