Significance: High-speed 3D imaging methods have been playing crucial roles in many biological discoveries. Aim: We present a hybrid light-field imaging system and image processing algorithm that can visualize high-speed biological events. Approach: The hybrid light-field imaging system uses the selective plane optical illumination, which simultaneously records a high-resolution 2D image and a low-resolution 4D light-field image. The high-resolution 4D light-field image is obtained by applying the hybrid algorithm derived from the deconvolution and phase retrieval methods. Results: High-resolution 3D imaging at a speed of 100-s volumes per second over an imaging field of 250 × 250 × 80 μm3 in the x, y, and z axis, respectively, is achieved with a 2.5 times enhancement in lateral resolution over the entire imaging field compared with standard light-field systems. In comparison to the deconvolution algorithm, the hybrid algorithm addresses the artifact issue at the focal plane and reduces the computation time by a factor of 4. Conclusions: The new hybrid light-field imaging method realizes high-resolution and ultrafast 3D imaging with a compact setup and simple algorithm, which may help discover important applications in biophotonics to visualize high-speed biological events.

中文翻译：

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高分辨率 3D 光场成像

意义：高速 3D 成像方法在许多生物发现中发挥着至关重要的作用。目的：我们提出了一种混合光场成像系统和图像处理算法，可以可视化高速生物事件。方法：混合光场成像系统使用选择性平面光学照明，同时记录高分辨率2D图像和低分辨率4D光场图像。高分辨率 4D 光场图像是通过应用源自反卷积和相位检索方法的混合算法获得的。结果：在 x、y 和 z 轴分别为 250 × 250 × 80 μm3 的成像场上以每秒 100 秒体积的速度实现高分辨率 3D 成像，使用 2. 与标准光场系统相比，整个成像场的横向分辨率提高了 5 倍。与反卷积算法相比，混合算法解决了焦平面的伪影问题，并将计算时间减少了 4 倍。 结论：新的混合光场成像方法实现了高分辨率和超快的 3D 成像，体积小巧设置和简单的算法，这可能有助于发现生物光子学中的重要应用，以可视化高速生物事件。