The utility of COI DNA barcodes in species delimitation is explored as well as life stage associations of five closely related Propsilocerus species: Propsilocerus akamusi (Tokunaga, 1938), Propsilocerus paradoxus (Lundström, 1915), Propsilocerus saetheri Wang, Liu et Paasivirta, 2007, Propsilocerus sinicus Sæther et Wang, 1996, and Propsilocerus taihuensis (Wen, Zhou et Rong, 1994). Results revealed distinctly larger interspecific than intraspecific divergences and indicated a clear “barcode gap”. In total, 42 COI barcode sequences including 16 newly generated DNA barcodes were applied to seven Barcode Index Numbers (BINs). A neighbor-joining (NJ) tree comprises five well-separated clusters representing five morphospecies. Comments on how to distinguish the larvae of P. akamusi and P. taihuensis are provided.

中文翻译：

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使用DNA条形码对Propsilocerus Kieffer，1923（Diptera，Chironomidae）进行物种界定和生命阶段关联

探索了COI DNA条形码在物种划界中的作用以及五个密切相关的Propsilocerus物种的生命周期关联：Propsilocerus akamusi（Tokunaga，1938年），Propsilocerus paradoxus（Lundström，1915年），Propsilocerus saetheri Wang，Liu et Paasivirta，2007年， Propsilocerus sinicusSæther等，Wang，1996年；太湖Propsilocerus taihuensis，（Wen，Zhou等，1994）。结果显示，种间差异明显大于种内差异，并表明存在明显的“条形码缺口”。总共将42个COI条形码序列（包括16个新生成的DNA条形码）应用于七个条形码索引号（BIN）。邻居连接树（NJ）包含五个分离良好的簇，代表五个形态种。提供了有关如何区分akamusi和taihuensis的幼虫的评论。