Male infertility is a global reproductive issue, several clinical approaches have been developed to tackle it, but their effectiveness is limited by the labour-intensive and time-consuming sperm selection procedures used. Here, we present an automated, acoustic based continuous-flow method capable of selecting high quality sperm with considerably improved motility and DNA integrity compared to the initial raw bull semen. The acoustic field translates larger sperm and guides highly motile sperm across the channel width. The result is the selection of sperm with over 50% and 60% improvement in vitality and progressive motility and more than 38% improvement in DNA integrity, respectively, while providing a clinically relevant volume and selected sperm number for the performance of in vitro fertilisation (IVF) and intracytoplasmic sperm injection (ICSI) by selecting over 60 000 sperm in under an hour.

中文翻译：

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使用表面声波选择高DNA完整性的精子

男性不育是全球性的生殖问题，已经开发出多种临床方法来解决，但其有效性受到所使用的劳动密集且费时的精子选择程序的限制。在这里，我们提出了一种基于声学的自动化连续流方法，与最初的原始牛精液相比，该方法能够选择具有显着改善的运动性和DNA完整性的高质量精子。声场转化较大的精子，并在通道宽度上引导高度活动的精子。结果是选择的精子的活力和进行性运动分别提高了50％和60％以上，DNA完整性提高了38％以上，同时提供了具有临床相关性的精子数量和选定的精子数量以用于体外表现 一个小时内选择超过60 000精子，进行受精（IVF）和胞浆内精子注射（ICSI）。