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Multimerized self-assembled caged two-in-one siRNA nanoparticles for photomodulation of RNAi-induced gene silencing
Chemical Science ( IF 7.6 ) Pub Date : 2020-10-12 , DOI: 10.1039/d0sc03562a Changmai Chen 1, 2, 3, 4, 5 , Nannan Jing 4, 5, 6, 7, 8 , Zhongyu Wang 4, 5, 6, 7, 8 , Yu Zhang 4, 5, 6, 7, 8 , Wei Chen 1, 2, 3, 4 , Xinjing Tang 4, 5, 6, 7, 8
Chemical Science ( IF 7.6 ) Pub Date : 2020-10-12 , DOI: 10.1039/d0sc03562a Changmai Chen 1, 2, 3, 4, 5 , Nannan Jing 4, 5, 6, 7, 8 , Zhongyu Wang 4, 5, 6, 7, 8 , Yu Zhang 4, 5, 6, 7, 8 , Wei Chen 1, 2, 3, 4 , Xinjing Tang 4, 5, 6, 7, 8
Affiliation
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We rationally designed and developed caged siRNA nanoparticles (Multi-Chol-siRNA) self-assembled with cholesterol-modified multimerized caged siRNAs for photomodulation of siRNA gene silencing activity. Strong resistance to serum nuclease and RNase A was observed for these cholesterol-modified caged siRNA nanoparticles due to the formation of nanostructures with high intensity of siRNA. These caged Multi-Chol-siRNA self-assembled nanoparticles were successfully used to achieve photochemical regulation of both exogenous GFP and endogenous Eg5 gene expressions with a GFP/RFP transient transfection system and Eg5-associated assays, respectively. Further, Two-in-One caged Multi-Chol-siGFP/siEg5 self-assembled nanoparticles simultaneously targeting GFP and Eg5 genes were also developed. The caged Multi-Chol-siRNA self-assembled nanoparticles have demonstrated the effectiveness of enhancing photomodulation of multiple RNAi-induced gene silencing activities in cells.
中文翻译：
多聚体自组装笼状二合一siRNA纳米粒子，用于光调制RNAi诱导的基因沉默。
我们合理地设计和开发了自组装的笼状siRNA纳米颗粒（Multi-Chol-siRNA），并与胆固醇修饰的多聚笼状siRNA自组装，以进行siRNA基因沉默活性的光调节。对于这些胆固醇修饰的笼状siRNA纳米颗粒，由于形成了具有高强度siRNA的纳米结构，因此观察到了对血清核酸酶和RNase A的强抗性。这些笼中的Multi-Chol-siRNA自组装纳米颗粒分别通过GFP / RFP瞬时转染系统和Eg5相关测定法，成功地用于对外源GFP和内源性Eg5基因表达进行光化学调节。此外，二合一还开发了同时靶向GFP和Eg5基因的笼状Multi-Chol-siGFP / siEg5自组装纳米颗粒。笼中的Multi-Chol-siRNA自组装纳米颗粒已证明增强细胞中多个RNAi诱导的基因沉默活性的光调节的有效性。
更新日期：2020-10-19
中文翻译：
多聚体自组装笼状二合一siRNA纳米粒子，用于光调制RNAi诱导的基因沉默。
我们合理地设计和开发了自组装的笼状siRNA纳米颗粒（Multi-Chol-siRNA），并与胆固醇修饰的多聚笼状siRNA自组装，以进行siRNA基因沉默活性的光调节。对于这些胆固醇修饰的笼状siRNA纳米颗粒，由于形成了具有高强度siRNA的纳米结构，因此观察到了对血清核酸酶和RNase A的强抗性。这些笼中的Multi-Chol-siRNA自组装纳米颗粒分别通过GFP / RFP瞬时转染系统和Eg5相关测定法，成功地用于对外源GFP和内源性Eg5基因表达进行光化学调节。此外，二合一还开发了同时靶向GFP和Eg5基因的笼状Multi-Chol-siGFP / siEg5自组装纳米颗粒。笼中的Multi-Chol-siRNA自组装纳米颗粒已证明增强细胞中多个RNAi诱导的基因沉默活性的光调节的有效性。
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